Induction of neutrophilic granulocytosis in mice by administration of purified human native granulocyte colony-stimulating factor (G-CSF)

Tamura, Masahiko; Hattori, Kunihiro; Nomura, Hitoshi; Oh-eda, Masayoshi; Kubota, Naoki; Imazeki, Ikuo; Ono, Masayoshi; Ueyama, Y; Nagata, Shinji; Shirafuji, Naoki; Asano, Shigetaka

doi:10.1016/0006-291x(87)90296-8

Cited by 126 publications

(56 citation statements)

References 10 publications

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“…Our rhG-CSF was produced in Chinese hamster ovary (CHO) cells and therefore glycosylated (26), while other groups used non-glycosylated rhG-CSF produced in Escherichia coli ( 1 1, 12). Actually, marked induction of erythroid colony forming units (CFU-E) and early erythroid progenitor cells (erythroid burst forming unit (BFU-E)) in spleen have been observed after repeated injection of the glycosylated rhG-CSF (9). In addition, the dose of rhG-CSF administered in the present experiment was higher than that used in a previous study ( 1 1).…”

Section: Discussioncontrasting

confidence: 55%

Effect of Recombinant Human Granulocyte Colony-Stimulating Factor on Survival in Lethally Irradiated Mice

et al. 1992

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Section: Discussioncontrasting

confidence: 55%

Effect of Recombinant Human Granulocyte Colony-Stimulating Factor on Survival in Lethally Irradiated Mice

et al. 1992

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“…It also increased the number of neutrophils in the blood and BALF. Several investigators have also demonstrated that G-CSF induces the recovery of normal neutrophil count in mice [3,17] or shortens the period of CPA-induced neutropenia in cynomolgus monkeys [18]. In addition, Matsumoto et al [3] also reported that morphologically mature neutrophils in G-CSF-treated mice caused elimination of Gram-positive and Gram-negative bacteria and Candida albicans.…”

Section: Discussionmentioning

confidence: 98%

Protective effect of granulocyte colony-stimulating factor (G-CSF) in a granulocytopenic mouse model of Pseudomonas aeruginosa lung infection through enhanced phagocytosis and killing by alveolar macrophages through priming tumour necrosis factor-alpha (TNF-α) production

Shirai

Kadota

Tomono

et al. 1997

Clinical and Experimental Immunology

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SUMMARYWe investigated the effects of G-CSF in a granulocytopenic mouse model of Pseudomonas aeruginosa lung infection. The model was prepared by intratracheal instillation of the bacteria, while granulocytopenia was induced by intraperitoneal injection of 4 . 0 mg of cyclophosphamide (CPA). There was no difference in the survival rate between G-CSF-treated animals and the normal group, and the number of neutrophils in the blood and lung recovered to normal in the former group. However, the phagocytic and killing activities of neutrophils were lower in G-CSF-treated mice than in controls. Interestingly, the mortality rate increased significantly when anti-TNF-a antibody was combined with G-CSF, although it was intermediate between CPA alone and CPA-G-CSF-treated mice. However, the improved mortality was not associated with a change in the number of neutrophils in the circulation and lung. Administration of anti-TNF-a antibody resulted in a significant suppression of TNF-a in bronchoalveolar lavage fluid and of enhanced alveolar macrophage function (phagocytic and bactericidal activity) against P. aeruginosa in G-CSF-treated granulocytopenic mice. We showed also increased TNF-a mRNA expression and TNF-a production in vitro using G-CSF-pretreated alveolar macrophages compared with control untreated macrophages. Our results are the first evidence to suggest that G-CSF provides a synergistic protective effect against lethal P. aeruginosa lung infection in the granulocytopenic host. This effect is probably due to enhancement of alveolar macrophage function through endogenous TNF-a production, in addition to increasing the number of circulating neutrophils.

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“…This phenomenon may help to explain the obvious quantitative discrepancy that exists between the weak proliferative action of G-CSF acting alone in vitro (6), and the effects of G-CSF in vivo, where major rises in neutrophil levels are readily inducible by G-CSF injection (10,11). It is of interest that G-CSF has only a very weak capacity to elevate neutrophil levels in W and Steel mutant mice (12), suggesting that the strong response elicited by G-CSF in normal mice and most humans may depend on an enhancement interaction with SCF.…”

mentioning

confidence: 99%

Direct proliferative actions of stem cell factor on murine bone marrow cells in vitro: effects of combination with colony-stimulating factors.

Metcalf

Nicola

1991

Proc. Natl. Acad. Sci. U.S.A.

149

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Stem cell factor (SCF), the ligand for the c-kit protooncogene product, was able to stimulate blast cell and granulocytic colony formation by precursors from normal murine bone marrow. The blast cell colonies contained a high content of progenitor cells able to form macrophage and/or granulocyte colonies. Clone transfer studies, the secondary culture of colony cells, and the culture of populations freed of accessory cells all indicated a direct proliferative action of SCF. SCF receptors were present in high numbers on blast cells and in lower numbers on immature granulocytic, monocytic, and eosinophilic cells. Combination of SCF with granulocyte, granulocyte-macrophage, or multipotential colony-stimulating factors, but not macrophage colony-stimulating factor, resulted in enhancement of colony size. Granulocyte colony-stimulating factor enhanced cell proliferation initiated by SCF, but not vice-versa, and resulted in a 10-fold increase in colony cell numbers and a 7-fold increase in progenitor cells in blast colonies. No evidence was obtained that SCF, alone or in combination with granulocyte colony-stimulating factor, could stimulate self-generation by blast colony-forming cells.

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Induction of neutrophilic granulocytosis in mice by administration of purified human native granulocyte colony-stimulating factor (G-CSF)

Cited by 126 publications

References 10 publications

Effect of Recombinant Human Granulocyte Colony-Stimulating Factor on Survival in Lethally Irradiated Mice

Effect of Recombinant Human Granulocyte Colony-Stimulating Factor on Survival in Lethally Irradiated Mice

Protective effect of granulocyte colony-stimulating factor (G-CSF) in a granulocytopenic mouse model of Pseudomonas aeruginosa lung infection through enhanced phagocytosis and killing by alveolar macrophages through priming tumour necrosis factor-alpha (TNF-α) production

Direct proliferative actions of stem cell factor on murine bone marrow cells in vitro: effects of combination with colony-stimulating factors.

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