Present study was carried out for the accuracy of commercially available progesterone ELISA kit at NCRP farm in the fiscal year 2015/16. Twenty crossbred Jersey and Holstein dairy cows were selected at different time periods of post insemination. Blood serum was collected in those animals and progesterone was quantified with the commercially available progesterone ELISA kit. Pregnancy diagnosis was performed by rectal palpation and Ultrasonography (USG) as a Gold standard and compared for the accuracy to ELISA kit. Results of the ELISA kit revealed the accuracy of the kit to be only 80 % with high sensitivity 92 % and very low specificity 57 % at 95 % confidence interval. Out of twenty artificially inseminated cows, thirteen were pregnant and seven were non-pregnant by the Gold standard test with their significantly different mean progesterone (at P < 0.05) 8.93±1.10 ng/ml and 4.36±1.21 ng/ml respectively. Hence, it can be used in the early pregnancy diagnosis at only after 24 days of the insemination, however, progesterone quantification by ELISA is not the confirmatory tests for the pregnancy diagnosis as this results accuracy of only 80 %.
Controlled internal drug release (CIDR) based hormonal protocols CoSynch + CIDR, OvSynch + CIDR and CIDR + PGF2α were applied to 25 crossbred anestrous Holstein and Jersey cows to improve the fertility at the farm of National Cattle Research Programme, Rampur, Chitwan. All three protocols were found equally effective (P>0.05) with 100% estrus expression rate, out of which, 80% (8/10), 85.7% (6/7) and 75% (6/8) had estrus expression with well cervix open at fixed time artificial insemination in CoSynch + CIDR, OvSynch + CIDR and CIDR + PGF2α protocols respectively and rest had estrus expression with partial cervix open. Statistically non-significant (P>0.05) pregnancy outcome that is 14.3 % (1/7) in CIDR+PGF2α group and 12.5 % (1/8) in OvSynch group while none in CoSynch + CIDR group were pregnant following fixed time artificial insemination. Poor pregnancy outcome of the anestrous cows in all protocols might not only have hormonal problem but also could have various other reasons which was beyond the objective of this research findings. Hence, study concludes anestrous cows respond well to the CIDR based hormonal protocols to revive the estrus.
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