Accuracy of Pregnancy Diagnosis with Commercially Available Progesterone Kit in Dairy Cows

Sah, Ashok Kumar; Bastola, R.; Pandeya, Y.R.; Pathak, Laxmi; Acharya, Madhav Prasad; Khanal, Doj Raj

doi:10.3126/nvj.v34i0.22908

Cited by 3 publications

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“…Upon measuring, its blood level concentration increases after ovulation and during pregnancy and reduces during follicular growth after corpus luteum regress and parturition [ 12 ]. Progesterone concentration is maintained by corpus luteum at a high level in pregnant cows compared with non-pregnant cows [ 5 ]. Hence, it is an ideal biomarker whose quantification could be helpful to determine whether the cow is cycling or is pregnant [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…There are no simple laboratory tests available to diagnose pregnancy in cattle. Rectal palpation is the oldest and most widely used method for early pregnancy diagnosis in dairy cattle and it has its own disadvantages of rectal bleeding, early embryonic mortality, and extreme stress on the pregnant animal and needs a skilled veterinarian [ 5 ]. Advanced methods currently available for pregnancy diagnose such as ultrasonography and radio immunoassay are developed and most used in developed countries and cannot be easily implemented in the rural areas of developing countries [ 6 ] such as Ethiopia as they are sophisticated and costly.…”

Section: Introductionmentioning

confidence: 99%

“…Progesterone detection for pregnancy diagnosis in farm animals is an important tool for early pregnancy diagnosis and infertility monitoring with high accuracy which contributes to increase economic efficiency of a farm [ 4 ]. Detection of progesterone concentration as a diagnostic tool has been made possible by the introduction of radioimmunoassay techniques [ 6 ] and enzyme-linked immunosorbent assay (ELISA) techniques [ 5 ]. This quantification could help in pregnancy examination as early after natural or artificial insemination [ 8 ], and almost 95% of cows with low progesterone will not be pregnant 24 days after AI [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Validation of the Human Progesterone Assay Kit for Cattle as a Pregnancy Diagnosis Tool

Lobago

Lemma

et al. 2022

Veterinary Medicine International

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Accurate pregnancy diagnosis is an important criterion and management tool for successful dairying. Early identification of non-pregnant dairy heifers and cows after breeding can improve pregnancy rate and life time production. Determination of progesterone hormone levels is more accurate to diagnose failed pregnancies in dairy animals. This method is not always available in developing countries. Some of the kits available are developed for humans and might be used for cattle because in principle, progesterone is not species-specific and detection methods are the same in both animals and human beings. The study aimed at validating a human progesterone ELISA kit for use in cattle as a pregnancy diagnosis tool. Forty Boran and crossbred cattle (22 pregnant and 18 non-pregnant) were selected for the study. Ten milliliter of blood sample was collected from each animal using jugular venipuncture. Serum I and plasma was harvested within 2 hours after venipuncture and serum II after 12 hours, and all samples were analyzed for progesterone concentration using the ELISA procedure provided with the kit. The result showed that 88.9% (n = 16) of non-pregnant cows had progesterone concentration below 1 ng/ml with mean (±SE) of 0.48 ± 0.75 ng/ml while all pregnant cows had mean (±SE) concentration of 19.3 ± 0.68 ng/ml with individual values ranging from 5.2–38 ng/ml. Progesterone concentration between breeds and sample type did not show statistically significant difference for pregnant and non-pregnant cows. Nonetheless, the results of the experiments are very promising as far as pregnancy diagnosis is concerned in dairy cows from an economic perspective and accuracy; the experiments have to be performed on larger scale to proof repeatability and sensitivity

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Validation of the Human Progesterone Assay Kit for Cattle as a Pregnancy Diagnosis Tool

Lobago

Lemma

et al. 2022

Veterinary Medicine International

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“…Until now, progesterone testing is considered the earliest method to diagnose pregnancy in cows. This method is 80% effective from day 21-24 post-insemination (Sah et al 2017;Srilatha et al 2017). Related research indicates examination time can be accelerated (Abdullah et al 2017;Siregar et al 2017;Srilatha et al 2017;Thasmi et al 2017;Widayati et al 2019).…”

Section: Introductionmentioning

confidence: 98%

Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations

et al. 2021

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This study aims to determine the effectiveness of IFN-? and progesterone measurement as the basis for pregnancy diagnosis in Aceh cows. The study used twelve cows aged 3-5 years, weighing 150-250 kg, clinically healthy and having normal reproduction. The estrous cycle was synchronized using prostaglandin hormone (LutalyseTM, Pharmacia & Upjohn Company, Pfizer Inc., 25 mg/cow, i.m.) with multiple injection protocol (10-day interval). Heat detection was conducted in the morning (08.00 Western Indonesia Time) and evening (16.00 Western Indonesia Time). Cows were inseminated 12-18 hours after standing heat. Blood was collected on day 4, 5, 6, 7, 14, 15, 16, 17, and 18 post-insemination. Concentrations of IFN-? and progesterone were determined by the ELISA method. Cows with an interferon concentration >6 pg/mL and a progesterone concentration >2 ng/mL were considered pregnant. Pregnancy data were then compared with transrectal ultrasound at day 25 as the gold standard. Concentrations of IFN-? in pregnant and non-pregnant cows were significantly different on day 18 post-insemination, while progesterone concentration was significantly different on day 15. The accuracy of IFN-? pregnancy diagnosis at peak IFN-? expression was obtained on day 18 post-insemination, with an accuracy rate of 66.7%, a sensitivity rate of 60% and a specificity rate of 71.4%. Meanwhile, the accuracy of progesterone-based pregnancy diagnosis was obtained on day 17 post-insemination, with an accuracy rate of 91.6%, a sensitivity rate of 83.3% and a specificity rate of 100%. It was concluded that the diagnosis of pregnancy using progesterone measurement was more effective than IFN-? measurement.

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“…If a cow is pregnant, P4 levels would not decrease on day 17 or 18, but would remain elevated (Petersson, 2007). Progesterone quantification in blood and milk in laboratory can be obtained by Radioimmunoassay (RIA) or Enzyme-Linked Immuno-Sorbent Assay (ELISA) methods (Sah et .al., 2017). This quantification could help in pregnancy examination as early as 21 days after natural or artificial insemination (Fricke et.al., 2016).…”

Section: Introductionmentioning

confidence: 99%

Pregnancy Diagnosis with Progesterone ELISA Kit in Farm Animals, Its Accuracy and Application

2019

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Pregnancy is a special condition where a female lodges one or more young ones within her uterus. It is maintained by various endocrine physiology and metabolic changes between maternal and fetal circumstances. Space dine secreted by corpus luteum increases extraordinarily throughout the pregnancy, estrogen increases rapidly during first trimester and prolonged lifespan of corpus luteum and small quantity of estrogen prevents prostaglandins pulsatic secretion. We reviewed available literature to evaluate the accuracy of progesterone ELISA test kit in pregnancy diagnosis in farm animals. We found varying accuracy in global and Nepalese context but found that this is an important tool for early pregnancy diagnosis and infertility monitoringwith high accuracy which contributes to increase economic efficiency of a farm. This kit has been used for qualitative and quantitative study of progesterone to understand the reproductive status of animals. Therefore, its use is increasing in globally and in Nepal. This kit has been used at various breeding centers, and livestock service centers of Nepal. However, wider level use is still difficult due to lack of infrastructuresand subsistence farming.

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Accuracy of Pregnancy Diagnosis with Commercially Available Progesterone Kit in Dairy Cows

Cited by 3 publications

References 0 publications

Validation of the Human Progesterone Assay Kit for Cattle as a Pregnancy Diagnosis Tool

Validation of the Human Progesterone Assay Kit for Cattle as a Pregnancy Diagnosis Tool

Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations

Pregnancy Diagnosis with Progesterone ELISA Kit in Farm Animals, Its Accuracy and Application

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