Accurate in situ leaf area index (LAI) estimates of forest plots are required to validate currently-used LAI map products. Woody-to-total area ratio ( α ) is a crucial parameter in converting the plant area index estimates of forest plots obtained by optical methods into LAI. Although optical methods for estimating the α of forest canopy have been proposed, their performance has never been assessed. In this study, five Larix gmelinii Rupr. forest plots with contrasting plot characteristics (i.e., tree age, tree height, management activities, stand density, and site conditions) were selected. The performance of two commonly used optical methods, namely, multispectral canopy imager (MCI) and digital hemispherical photography (DHP), in estimating the α of L. gmelinii forest plots was evaluated by using the reference α of the selected forest plots. The reference α of forest plots was measured via destructive method by harvesting two or three representative trees in each plot. Large variations were observed amongst the reference α of the selected forest plots (ranging from 0% to 56%). These α were also highly correlated with the site conditions and management activities in these plots. The effective α ( α e ) or α estimated using the leaf-on and leaf-off periods MCI or DHP images with or without consideration of the clumping effects of canopy element and woody components were 1.57 to 4.63 times the reference α in the five plots. The overestimation of α or α e was mainly caused by the preferential shading of woody components by the shoots in the leaf-on canopy. Accurate α estimates for the L. gmelinii forest plots with errors of less than 20% can be obtained from MCI when the clumping effects of canopy element and woody components are considered in the estimation.
Somatic hybrid cell lines were constructed by the fusion of protoplasts isolated from cell suspensions of Zea mays L. (maize, 2n = 20) and Triticum sect, trititrigia MacKey (trititrigia, 2n = 35), a perennial hybrid of T. durum Desf. and Elytrigia intermedium (Host) Nevski. Iodoacetamide-inactivated protoplasts of maize were fused with trititrigia protoplasts, which were sensitive to the PEG/DMSO fusion treatment at high pH and high calcium. Based on physiological complementation, approximately 0.002% of the total protoplasts cultured following fusion treatment developed into cell colonies, and 79 lines of them, almost a half, were singled out and subcultured. Among the subcultured lines three were, in comparison with the parents, identified as somatic hybrids by their coupled XbaI restriction patterns of total DNAs probed with the ribosomal DNA of rice. Southern analysis of the digested total DNAs with a mitochondrial gene, atpA., from pea, or a chloroplast gene, trnK, from rice, revealed that all the hybrids carried only the organellar DNAs of trititrigia, which excluded the possibilities of a chimeric callus or any DNA contamination. Cytogenetically, one hybrid was mixoploid with a 2n of 46-67 in which chromosomal endoreduplication, characterized by the appearance of diplochromosomes, was occasionally observed. Its hybridity was reconfirmed by the fact that it bore the satellite chromosomes of both maize and trititrigia, which were distinguishable from each other by size. In contrast, the other two hybrids were aneuploids. The potential of gene transfer between Zea and Triticum species was thus conclusively established.
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