The purpose of the study was to investigate the role of flavonoids from Emilia sonchifolia (ES) on the progression of selenite-induced cataract. The antioxidant property of the flavonoids isolated from ES was assessed by measuring its capacity to inhibit superoxide production and serum oxidation in vitro in comparison with quercetin. Based on these experiments, an in vivo study was conducted to evaluate the modulatory effects of the flavonoids against selenite cataract. Cataract was induced by a single subcutaneous injection of sodium selenite (4 mg/kg body weight). The treatment group received flavonoids from ES (1 mg/kg) and this was compared with the quercetin treated group. Lens opacification was monitored by a slit lamp microscope and classified into six stages. Activity of the antioxidant enzymes - superoxide dismutase and catalase - and the level of lipid peroxidation products thiobarbituric acid reacting substances and reduced glutathione were studied. Slit lamp examination showed that the flavonoid fraction from ES could modulate the progression of cataract. Activities of superoxide dismutase, catalase and reduced glutathione were found to be increased in the ES treated groups, while thiobarbituric acid reacting substances were decreased compared with the selenite-induced group. The results suggest that flavonoids from ES can modulate lens opacification and oxidative stress in selenite-induced cataract.

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Dregea volubilis is a woody climbing plant commonly found in the hotter parts of India. The leaves are edible and used as a green vegetable, while the plant extract has been used traditionally to treat several diseases including eye ailments. Drevogenin D is a triterpenoid aglycone that has been isolated, purified, and characterized as an active component from the leaves of D. volubilis. In this study, drevogenin D was evaluated for antioxidant and potential anticataractogenic activity in an in vitro model. 1,1-Diphenyl-2-picrylhydrazyl radical and superoxide radical scavenging activities of drevogenin D were studied and found to exhibit a 50% inhibitory concentration of 43 microg/mL and 200.6 microg/mL, respectively. Normal rat lenses cultured in 0.1 mM sodium selenite-supplemented medium were used as the experimental model for this study. Selenite-induced models are excellent mimics of oxidative stress induced cataract. Treatment with drevogenin D at a concentration of 50 microg/mL medium was found to reverse the level of activity of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, elevate the level of reduced glutathione and protein sulfhydryl, and lower the level of lipid peroxidation as indicated by the concentration of thiobarbituric acid-reacting substances. These results indicate good antioxidant activity and potential anticataractogenic activity for drevogenin D against selenite-induced cataractous changes, which have been reported for the first time.

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