Saraca asoca (Family Caesalpiniaceae) has been widely used in traditional Indian medicine especially due to its wound-healing property. The present study investigates the chemopreventive property of flavonoids from Saraca asoca (flowers) on 2-stage skin carcinogenesis in mice models. Skin cancer was induced in Swiss albino mice by single topical application of 7,12-dimethyl benzanthracene (100 µg/50 µL of acetone) followed by thrice a week treatment of croton oil for 20 weeks. The topical pretreatment of the flavonoid fraction from S asoca (FF S asoca) was 30 minutes prior to the application of croton oil thrice weekly for 20 weeks. At the end of the experimental period the animals were sacrificed, and the tumor statistics and various marker parameters were studied (enzyme assays, Western blotting). The pretreatment of the FF of S asoca caused significant reduction in the number of tumors per mouse and the percentage of tumor-bearing mice. Also, the latency period for the appearance of the first tumor was delayed by S asoca pretreatment. In plant-treated animals there was a significant increase in the levels of reduced glutathione, catalase, and protein in skin when compared with the untreated animals. Conversely, there was a significant decrease in the lipid peroxidation levels. A significant reduction in the expression of ornithine decarboxylase, a key enzyme in the promotion stage of 2-stage skin cancer, in the plant-treated group was also observed. These findings suggest the chemopreventive activity of flavonoids from S asoca on 2-stage skin carcinogenesis.
Saraca asoka (Family - Caesalpiniaceae) has been widely used in the Ayurvedic (traditional Indian) system of medicine especially due to its wound healing property. The present study investigated the chemopreventive property of flavonoids from the flowers of Saraca asoka on 7,12 dimethyl benz(a)anthracene (DMBA) induced skin cancer in mice models. A single topical application of DMBA (100 microg/50 microL of acetone) followed after 2 weeks by three times a week treatment with croton oil (1% in acetone), for 20 weeks resulted in tumor induction. The topical application of the flavonoid fraction of S. asoka (FF S. asoka), 30 min prior to the application of croton oil thrice weekly for 20 weeks, caused a significant reduction in the number of tumors per mouse and the percentage of tumor-bearing mice. Also the latency period for the appearance of the first tumor was delayed by S. asoka pretreatment. In the flavonoid fraction (5 mg and 10 mg/kg body weight) treated animals, the levels of biochemical markers - rhodanese, myeloperoxidase, beta-D-glucuronidase, sialic acid, hexokinase and caspase 3 were significantly restored to near normal levels. These findings suggest the chemopreventive activity of flavonoids from S. asoka on two stage skin carcinogenesis. Histological data also support the chemopreventive potential of S. asoka.
105 hyperdopaminergic state can lead on to schizophrenia (37). A membrane fluidity dependent inhibition of Na'-Kf ATPase can lead on to a paroxysmal depolarisation shift and epilepsy (38). Thus hypomagnesemia consequent to hypothalamic digoxin and elevated dolichol can alter glycoconjugate metabolism in neuropsychiatric disorders.
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