The growth of III-nitride thin films with a high quality including few defects is essentially required for many applications, such as optical devices, high frequency devices, and high power devices [1,2]. The III-nitride compound semiconductors are a tetrahedrally coordinated binary compounds, found in either cubic zinc-blende (ZB) or hexagonal wurtzite (WZ) structures [3]. The two structures, ZB and WZ, differ in the relative handedness of the fourth interatomic bond along the (111) chain. The layer stacking sequence for WZ is ABABABA…, and that of ZB is ABCABCA…, along (111), respectively [3,4]. The epitaxial growth of AlN and GaN thin films on silicon (Si) substrates is very attractive, as it has several advantages, such as high quality and large area, compared with compound substrates. In addition, nitride-on-silicon structures afford the possibility of an excellent candidates for unique design architectures and for creating devices for high-power applications. Therefore, much effort has been concentrated on the growth of AlN and GaN layers on Si substrates [5][6][7].For the epitaxial growth of nitride thin films, a Si (110) wafer has begun to attract attention as a substrate, due to its interesting interface structure [5,8,9]. When nitrides are grown on a Si (110) substrate, the orientation relationship between the GaN and AlN and the Si is The main purpose of this study was to understand microstructural properties, such as the atomic structure, dislocation distribution, and strain behaviors, of GaN/AlN structures grown on Si (110) substrates. The microstructural properties of the GaN/AlN structures were studied in detail using TEM. Specifically, the interface structures between the GaN, AlN and the substrate and the dislocation behavior at the interfaces were detailedly studied using bright-field and dark-field (BF and DF) TEM images taken under two-beam conditions and HRTEM micrographs. In order to study the strain behavior at the interface, geometrical phase analysis (GPA) was conducted using the GPA for Gatan DigitalMicrograph program from HREM Research Inc.The schematic diagram of GaN/AlN/Si (110) structures was shown in Fig. 1(a). The analysis of the SAED patterns in Fig. 1(b) indicates that the orientation relationship between the WZ structure of the AlN and the Si (110) _ 20] zone axis around a tilting axis perpendicular to the growth surface. By applying the invisibility criterion, g⋅b=0, where g is a diffraction vector and b is a dislocation Burgers vector, it is likely that the dislocations in Figs. 2(a) 1576