Aim:The study was conducted to evaluate eight tree leaves based on polyphenolic content and rumen in vitro incubation and gas production technique (RIVIGPT) for their nutritive potentiality.Materials and Methods:Eight selected tree leaves, namely Sesbania grandiflora, Melia dubia, Dillenia spp., Artocarpus heterophyllus, Commiphora caudata, Moringa oleifera, Leucaena leucocephala, and Acacia auriculiformis, were selected for proximate composition, forage fiber fractions, total phenolics (TPs), non-tannin phenols (NTPs), total tannins (TTs), condensed tannins (CTs), and hydrolysable tannins (HTs); RIVIGP with and without polyethylene glycol (PEG); and in vitro dry matter digestibility (IVDMD) (modified in vitro two stage) analysis was conducted. On the basis of RIVIGPT, the in vitro digestible organic matter (IVDOM) and dry matter intake (DMI) was calculated.Results:Crude protein (CP) content of tree leaves ranged from 9.59 to 25.81%, neutral detergent fiber (NDF) 28.16 to 53.33%, acid detergent fiber (ADF) 21.26 to 41.7%, acid detergent lignin (ADL) 3.62 to 21.98%, TP 1.83 to 17.35%, TT 0.40 to 15.47%, and CTs 0.02 to 15.26%. IVDMD (%) was ranged from 64.95 to 88.12. The mean metabolizable energy (ME) (MJ/Kg) of tree leaves estimated with and without PEG was 7.75±0.56 and 8.75±0.39, in vitro gas production at 24 h (IVGP24) (ml) 31.06±4.14 and 37.09±2.64, initial gas production (a) (ml) 0.49±0.63 and 1.33±0.72, potential gas production (D) (ml) 38.74±4.27 and 43.79±2.44, rate of gas production (k) (h−1) 0.11±0.02 and 0.11±0.013, t1/2(ml) 9.81±2.41 and 7.42±0.80, in vitro gas production at 96 h IVGP96 (ml) 39.50±4.430 and 45.14±2.65, the predicted IVDOM (%) 55.44±4.15 and 61.98±3.03, and DMI (g/Kg W0.75) 103.1±14.76 and 104.3±10.16, respectively. The addition of PEG showed an improvement in IVGP24, IVGP96, ME, predicted IVDOM, and predicted DMI. CP was positively correlated with ME, IVGP24, IVGP96, a+b, k (r=0.749, p<0.05), IVDMD, IVDOM, and DMI (r=0.838, p<0.05) and negatively correlated with a and t1/2. NDF, ADF, and ADL contents were negatively correlated with ME (r=0.899, p<0.05), IVGP24 (r=−0.867, p<0.05), IVGP96 (r=−0.858, p<0.05), a+b (p<0.05), k (r=−0.828, p<0.05), IVDMD, IVDOM (r=−0.853, p<0.05), and DMI and positively correlated with a and t1/2. TP, TT, and CT were negatively correlated with ME, IVGP, IVGP96, a+b, k, IVDMD, IVDOM, and DMI and positively correlated with a (r=0.808, p<0.05) and t1/2. ME (MJ/Kg) was positively correlated with IVGP24 (r=0.938, p<0.05), IVGP96 (r=0.875, p<0.05), a+b (r=0.813, p<0.05), k (r=0.731, p<0.05), IVDMD, IVDOM (r=0.985, p<0.05), and DMI (r=0.727, p<0.05) and negatively correlated with a and t1/2.Conclusion:In the present study, the potentiality of tree leaves was assessed based on CP, ADF, ADL, TP, CT, IVGP, ME, IVDMD, predicted IVDOM, and predicted DMI. Based on this, it can be concluded that S. grandiflora, M. dubia, M. Oleifera, and L. leucocephala were graded as best; A. heterophyllus and C. caudata as moderate; and Dillenia spp. and A. auriculiformis as lowest pot...
India is the highest milk producing country in the world. The milk production in Kolar and Chikkaballapur districts had also increased in the last few years. In a view to assess the increase in milk production, a study on milk production trends was undertaken at Kolar and Chikkaballapur districts. A total of 120 dairy farmers were randomly selected and data were collected using an interview schedule. The secondary data were obtained from Kolar Milk Union Limited. The compound growth of volume of milk procurement during 1998-99 to 2011-12 is 4 per cent and seasonality in milk procurement was highest in the months of October and November and lowest in April and March. Suitable extension strategies should be developed in order to curb the fluctuation in milk production, this fluctuation may be due to unavailability of feed and green fodder, so suitable strategies should be taken to encourage conservation of fodder and to make it available throughout the year.
Background: Mudhol Hound the popular dog breed of Karnataka State, India has a long historical association with wars, hunting, social bondage, guarding and recently with the Indian army as a part of army’s sniffer dog squad. Despite its unique qualities, the number of animals is getting reduced gradually. Hence the present study was aimed at molecular characterization of the Mudhol Hound which will be useful in maintenance of diversity of the breed. Methods: In this study, 26 microsatellite markers were used for the characterization of Mudhol Hound dog. A total of 50 blood samples were screened. Microsatellite loci were analyzed by running the PCR products on 6.5 per cent polyacrylamide gels followed by silver staining. Genotyping was done based on the banding patterns. Result: A total of 221 alleles were observed from the 26 microsatellites. The number of alleles ranged from 5 (AHTk211) to 12 (INU055, PEZ12) with a mean of 8.5±1.9442 alleles. The average H0 and He were 0.7685±0.2122 and 0.8326±0.0397, respectively. The percentage of polymorphic loci obtained was 100 as all the loci studied were found to be polymorphic having the PIC values more than 0.5. All the 26 loci showed significant deviation from Hardy - Weinberg equilibrium (P less than 0.001).
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