A rod-shaped, Gram-positive bacterium, strain TNJL143-2 T , having N-acyl-D-amino acid amidohydrolase (D-aminoacylase) activity, was isolated from a soil sample from Natori, Japan. It was a non-spore-forming, strictly aerobic bacterium without motility, showing a temperature optimum for growth of 30 6C and a pH optimum for growth of 5-7. The 16S rRNA gene sequence of the strain showed the highest similarities to members of the genus Microbacterium, in particular, Microbacterium aerolatum, Microbacterium foliorum and Microbacterium phyllosphaerae. The chemotaxonomic characteristics, including the compositions of cellular menaquinones, cellular fatty acids and cell-wall amino acids, were consistent with those described for the genus Microbacterium. The G+C content of the genomic DNA was determined as 69?1 mol%. DNA-DNA hybridization studies using type strains of M. aerolatum, M. foliorum and M. phyllosphaerae showed only low levels of relatedness (11-12 %). On the basis of these phenotypic and genotypic results, a novel species, Microbacterium natoriense sp. nov., is proposed, with TNJL143-2 T (=JCM 12611 T =ATCC BAA-1032 T ) as the type strain.Members of the genus Microbacterium, in which two genera formerly designated Microbacterium and Aureobacterium have been unified (Takeuchi & Hatano, 1998a, b), are widespread in nature, and some species of this genus are of significance in biotechnology, e.g. the amino acid industry (Nakayama & Soda, 1998). During the course of our studies to screen micro-organisms that were able to grow on N-acetyl-D-phenylalanine as the sole nitrogen and carbon source, we found that a yellow-pigmented bacterium, strain TNJL143-2 T , isolated from soil at Natori, Miyagi, Japan, produced a novel N-acyl-D-amino acid amidohydrolase (D-aminoacylase). As a result, extensive physiological, chemotaxonomic and phylogenetic analyses were carried out. On the basis of these results, we propose that strain TNJL143-2 T represents a novel species of the genus Microbacterium, which we have named Microbacterium natoriense sp. nov. General laboratory cultivation was performed using LB broth (5 ml) at 30 uC. Bacterial growth was monitored for up to 7 days after inoculation by measuring the turbidity, at 600 nm, of cultures in 25 ml test tubes, which were capped with air-permeable silicone plugs and incubated in a water-bath incubator. For measurement of turbidity, an uninoculated control was used as a blank. Cell morphology was observed by light microscopy after 24 h cell growth. Sporulation was observed by phase-contrast microscopy after the cells had reached stationary phase. Gram stainingThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TNJL143-2 T is AY566291. was carried out using exponentially growing cells, according to Hucker's modification (Cowan & Steel, 1965), with reagents (Favour G) produced by Nissui. Flagellation was examined using Leifson's method (Cowan & Steel, 1965). The temperature range for growth of the organism was examined at 4 u C and at 10-50 u C in 5 ...
SUMMARY A 65-year-old man had diffuse, bilateral corneal oedema after acute intake of alcohol. Three days later the total opacification began to clear from the peripheral area towards the centre of the cornea. One month later both corneas were completely clear, and visual acuity was 20/20 in both eyes. Specular microscopy showed in the right eye greatly enlarged endothelial cells, which subsequently shrank, and they showed some pleomorphism six months later. The pleomorphic endothelial cells in the left eye changed little with time. Our findings suggested that the corneal oedema in this patient resulted from temporary endothelial dysfunction with extensive cell loss.It is well known that the acute intake of alcohol causes a disturbance of vision related to damage of the optic nerve.' To our knowledge no reports have described temporary corneal oedema from acute ingestion of alcohol. We describe such a case in a 65-year-old Japanese man. Case reportA 65-year-old man drank about 10 1 of distilled Japanese sake on 15 October 1984 and lost conCorrespondence to T Shiono, MD. sciousness. The next day he was admitted to hospital for continued unconsciousness, dyspnoea, and cyanosis. Hypoglycaemia (16 mg/dl (089 mmol/l) and an elevated aspartate transaminase level (258 KU) were found. The results of the following tests were normal: renal function, haemogram, and blood ammonium concentration. No signs of infection were found.On a diagnosis of hypoglycaemia-induced unconsciousness the patient was given an intravenous drip injection of glucose and oxygen inhalation. His blood glucose concentration increased to 104 mg/dl (5-8
A 6-year-old girl had bilaterally subluxated lenses and liver and platelet dysfunctions. A cyanide-nitroprusside test of the urine was positive. Serum and urinary levels of methionine and homocystine were high. Cystathionine synthetase activity in the cultured skin fibroblasts was negligible. Pyridoxine, 1000 mg/day orally, normalized the serum and urinary methionine and homocystine levels and the liver and platelet functions. Episodes of pupillary block glaucoma prompted lens extraction. The subluxated lenses were extracted under general anaesthesia after administration of pyridoxine and dextran to avoid thrombosis. A good result without serious complications was obtained. We believe that homocystinuria should not be considered a contra-indication to general anaesthesia. In the pyridoxine-responsive patient general anaesthesia is not hazardous if platelet function can be controlled by the vitamin.
Single-phase Ba3La2LuCu6O y (y∼14) is synthesized. Superconductivity is observed after annealing in oxygen at high pressure; it is a semiconductor without annealing. Power X-ray diffraction shows that annealing changes the crystal structure of Ba3La2LuCu6O y from tetragonal into orthorhombic. Magnetic susceptibility measurements show that T c onset of the annealed sample is around 50 K and about 1% of the volume is superconductive. The analysis shows that the average Cu valence dependence on T c of Ba3La2LuCu6O y and Ba2YCu3O7-y differs.
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