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MicroRNAs (miRNAs) are known to fine‐tune growth, development, and stress‐induced responses. Osa‐miR1873 is a rice‐specific miRNA targeting LOC_Os05g01790. Here, we show that Osa‐miR1873 fine‐tunes rice immunity against Magnaporthe oryzae and yield traits via LOC_Os05g01790. Osa‐miR1873 was significantly upregulated in a susceptible accession but downregulated in a resistance accession at 24 h post‐inoculation (hpi) of M. oryzae. Overexpressing Osa‐miR1873 enhanced susceptibility to M. oryzae and compromised induction of defense responses. In contrast, blocking Osa‐miR1873 through target mimicry compromised susceptibility to M. oryzae and enhanced induction of defense responses. Altered expression of Osa‐miR1873 also resulted in some defects in yield traits, including grain numbers and seed setting rate. Moreover, overexpression of the target gene LOC_Os05g01790 increased rice blast disease resistance but severely penalized growth and yield. Taken together, we demonstrate that Osa‐miR1873 fine‐tunes the rice immunity‐growth trade‐off via LOC_Os05g01790, and blocking Osa‐miR1873 could improve blast disease resistance without significant yield penalty. Thus, the Osa‐miR1873‐LOC_Os05g01790 regulatory module is valuable in balancing yield traits and blast resistance.

MicroRNAs (miRNAs) play essential roles in rice immunity against Magnaporthe oryzae, the causative agent of rice blast disease. Here we demonstrate that Osa-miR162a fine-tunes rice immunity against M. oryzae and yield traits. Overexpression of Osa-miR162a enhances rice resistance to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H 2 O 2). In contrast, blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162a enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H 2 O 2 accumulation. Moreover, the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in slight reduced yield per plant, whereas the transgenic lines blocking Osa-miR162 show an increased number of grains per panicle, resulting in increased yield per plant. Altered accumulation of Osa-miR162 had a limited impact on the expression of rice Dicer-like 1 (OsDCL1) in these transgenic lines showing normal gross morphology, and silencing of OsDCL1 led to enhanced resistance to blast fungus similar to that caused by overexpression of Osa-miR162a, suggesting the involvement of OsDCL1 in Osa-miR162a-regulated resistance. Together, our results indicate that Osa-miR162a is involved in rice immunity against M. oryzae and fine-tunes resistance and yield.

ORCID IDs: 0000-0002-6747-4302 (J.F.); 0000-0003-4066-4875 (W.-M.W.).Circular RNAs (circRNAs) play roles in various biological processes, but their functions in the rice (Oryza sativa) response to Magnaporthe oryzae remain elusive. Here, we demonstrate that circRNAs are involved in the rice-M. oryzae interaction using comparative circRNA-sequencing and transgenic approaches. We identified 2932 high-confidence circRNAs from young leaves of the blast-resistant accession International Rice Blast Line Pyricularia-Kanto51-m-Tsuyuake (IR25) and the blast-susceptible accession Lijiangxin Tuan Heigu (LTH) under M. oryzae-infected or uninfected conditions; 636 were detected specifically upon M. oryzae infection. The circRNAs in IR25 were significantly more diverse than those in LTH, especially under M. oryzae infection. Particularly, the number of circRNAs generated per parent gene was much higher in IR25 than in LTH and increased in IR25 but decreased in LTH upon M. oryzae infection. The higher diversity of circRNAs in IR25 was further associated with more frequent 39 and 59 alternative back-splicing and usage of complex splice sites. Moreover, a subset of circRNAs was differentially responsive to M. oryzae in IR25 and LTH. We further confirmed that circR5g05160 promotes rice immunity against M. oryzae. Therefore, our data indicate that circRNA diversity is associated with different responses to M. oryzae infection in rice and provide a starting point to investigate a new layer of regulation in the rice-M. oryzae interaction.

Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression. Front. Genet. 11:327.

Rapid urbanization and intensification of human activities increases the risk of disturbance of ecological systems via multiple sources, with consequences for regional ecological security and health. Landscape ecological risk assessment (LERA) is an effective way to identify and allocate risk to resources. We used the north and south Qinling Mountain area as a case study to analyze the spatial heterogeneity of landscape ecological risk using a potential- connectedness-resilience three-dimensional (PCR 3D) framework based on an integrated and dynamic risk assessment concept from adaptive cycle theory. We explored factors driving the risks with a spatial model GeoDetector. The results show that the comprehensive landscape ecological risk was north–south polarized and dominated by low and moderate risk levels (90.13% of total risk) across the whole study area. The high-risk area was centered on the Weihe plain north of the Qinling Mountains (NQL), while low-risk areas accounted for 86.87% of the total area and were prevalent across the south of the study area. The areas with high potential and connectedness risks were centered in the Xi’an–Xianyang urban agglomeration and those with high-resilience risk were in the upper reaches of the Hanjiang River. The vast majority of the area to the south of the Qinling Mountains (SQL) is at low risk. In terms of driving forces, population density and vegetation coverage (NDVI) are the primary factors affecting landscape ecological risk. Our findings suggest that anthropogenic activity is the primary cause of landscape ecological risks in the study area and regional socioeconomic exploitation and environmental conservation need to be rebalanced to achieve sustainability for the social ecosystem. The PCR 3D LERA framework employed in this study can be used to inform landscape ecological health and security and to optimize socioeconomic progress at regional scales.

Global urbanization has led to drastic land use change, interfering the ecosystem services (ES) supply-demand balance, in turn threatening the well-being of humans. However, existing studies mainly stranded at the historical and current analysis, and the effects of urban spatial expansion on the relationship between ES supply and demand in the future are less clear, in particular at an urban agglomeration scale. This study was constructed with a framework of assessing the effects of urban spatial expansion on ES supply-demand mismatching under different future scenarios in the Guanzhong Plain Urban Agglomeration (GPUA) by using the Future Land Use Simulation (FLUS) model and expert-based Land-Use and Land-Cover Change (LUCC) matrix. The results showed that: (1) Urban expansion is significant in the natural development (ND) scenario, mainly manifesting the great transfer of dry land to construction land. (2) The gap between total ES supply and demand is narrowed from 2000 to 2030 and the mismatch between ES supply and demand is mainly reflected in the spatial distribution pattern in the GPUA. The ES budgets were in high surplus in Northern Qinling Mountains and northeast mountain areas, while they were in severe deficit in urban center areas. The budgets deficit under the ND scenario in 2030 is the most severe. (3) The gradient differences of ES budgets of the GPUA between urban centers and suburbs increase from 2000 to 2030 under two scenarios. The deficit region expands largest under ND scenario. The findings revealed that ES declining and supply-demand mismatching were triggered by the drastic land-use change driven by rapid urban expansion. The expansion has brought about an increasing material demand and growing industries, threatening the sustainability of ecosystems. Scenarios setting could contribute to coordinating the relationship between future urban development and ecological protection, and the policy strategies proposed in the

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