The regulation mechanism of the hybrid yellow cat sh "Huangyou-1" was assessed under conditions of hypoxia and reoxygenation by examination of oxygen sensors and by monitoring respiratory metabolism, oxidative stress, and apoptosis. The expressions of genes related to oxygen sensors (HIF-1α, HIF-2α, VHL, HIF-1β, PHD2, and FIH-1) were upregulated in the brain and liver during hypoxia, and recovered compared with control upon reoxygenation. The expressions of genes related to glycolysis (HK1, PGK1, PGAM2, PFK, and LDH) were increased during hypoxia and then recovered compared with control upon reoxygenation. The expressions of CS and SDH were lower than those of control during hypoxia and increased upon reoxygenation. Under hypoxic conditions, the expressions of genes related to oxidative stress (SOD1, SOD2, GSH-Px, and CAT) and the activity of antioxidant enzymes (SOD, CAT, and GSH-Px) and MDA were upregulated compared with control. The expressions of genes related to apoptosis (Apaf-1, Bax, Caspase 3, Caspase 9, and p53) were higher than those in control during hypoxic exposure, while the expressions of Bcl-2 and Cyt C were decreased. The ndings of the transcriptional analyses will provide insights into the molecular mechanisms of hybrid yellow cat sh "Huangyou-1" under conditions of hypoxia and reoxygenation.
Suppressors of cytokine signaling (SOCS) are a family of intracellular proteins emerging as key physiological regulators of cytokine responses in fish innate immune system. In this study, the tissue-specific distribution indicated that PfSOCS1, 2 and 3 were expressed ubiquitously and differentially in eight examined tissues. The highest transcript levels of PfSOCS1 and 3 respectively expressed in gill and liver, while that of the PfSOCS2 appeared mainly in muscle, followed by head kidney. The temporal patterns of PfSOCSs were assessed through the experimental challenge of A. hydrophila or E. ictaluri, and their expressions were altered in liver, gill and head kidney. Concretely, western blotting and quantitative real-time PCR analyses showed that PfSOCSs were significantly up-regulated in the early stage and then decreased after A. hydrophila or E. ictaluri challenge. Furthermore, the innate immune response of PfSOCSs in gill was more sensitive than that in liver and head kidney. PfSOCSs played vital roles in response to A. hydrophila or E. ictaluri challenge in three essential immune-related tissues. Our findings suggested that PfSOCSs played crucial roles in innate immunity of P. fulvidraco, and provided useful evidence for further understanding on the modulation mechanism of PfSOCSs in the innate immune system of P. fulvidraco
The regulation mechanism of the hybrid yellow catfish “Huangyou-1” was assessed under conditions of hypoxia and reoxygenation by examination of oxygen sensors and by monitoring respiratory metabolism, oxidative stress, and apoptosis. The expressions of genes related to oxygen sensors (HIF-1α, HIF-2α, VHL, HIF-1β, PHD2, and FIH-1) were upregulated in the brain and liver during hypoxia, and recovered compared with control upon reoxygenation. The expressions of genes related to glycolysis (HK1, PGK1, PGAM2, PFK, and LDH) were increased during hypoxia and then recovered compared with control upon reoxygenation. The expressions of CS and SDH were lower than those of control during hypoxia and increased upon reoxygenation. Under hypoxic conditions, the expressions of genes related to oxidative stress (SOD1, SOD2, GSH-Px, and CAT) and the activity of antioxidant enzymes (SOD, CAT, and GSH-Px) and MDA were upregulated compared with control. The expressions of genes related to apoptosis (Apaf-1, Bax, Caspase 3, Caspase 9, and p53) were higher than those in control during hypoxic exposure, while the expressions of Bcl-2 and Cyt C were decreased. The findings of the transcriptional analyses will provide insights into the molecular mechanisms of hybrid yellow catfish "Huangyou-1" under conditions of hypoxia and reoxygenation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.