Studies of Acute Hypoxia and Reoxygenation on Oxygen Sensors, Respiratory Metabolism, Oxidative Stress, and Apoptosis in Hybrid Yellow Catfish "Huangyou-1"

Abstract

The regulation mechanism of the hybrid yellow catfish “Huangyou-1” was assessed under conditions of hypoxia and reoxygenation by examination of oxygen sensors and by monitoring respiratory metabolism, oxidative stress, and apoptosis. The expressions of genes related to oxygen sensors (HIF-1α, HIF-2α, VHL, HIF-1β, PHD2, and FIH-1) were upregulated in the brain and liver during hypoxia, and recovered compared with control upon reoxygenation. The expressions of genes related to glycolysis (HK1, PGK1, PGAM2, PFK, and LDH) were increased during hypoxia and then recovered compared with control upon reoxygenation. The expressions of CS and SDH were lower than those of control during hypoxia and increased upon reoxygenation. Under hypoxic conditions, the expressions of genes related to oxidative stress (SOD1, SOD2, GSH-Px, and CAT) and the activity of antioxidant enzymes (SOD, CAT, and GSH-Px) and MDA were upregulated compared with control. The expressions of genes related to apoptosis (Apaf-1, Bax, Caspase 3, Caspase 9, and p53) were higher than those in control during hypoxic exposure, while the expressions of Bcl-2 and Cyt C were decreased. The findings of the transcriptional analyses will provide insights into the molecular mechanisms of hybrid yellow catfish "Huangyou-1" under conditions of hypoxia and reoxygenation.

Profiling Genetic Breeding Progress in Bagrid Catfishes

“…In reciprocal crosses of P. fulvidraco × P. vachelli, the hybrid combination of P. fulvidraco ♀× P. vachelli ♂demonstrates survival rates, fertility rates, and emergence rates exceeding 86.5%, 87.5%, and 71.6%, respectively [86][87][88] On the other hand, the hybrid combination of P. vachelli ♀× P. fulvidraco ♂exhibits rates of 79%, 53%, and 6.9% [89]. The cross group displays high heterosis, fast growth rates, and significant absolute weight gain, while the backcross group is unsuitable for production due to low fertilization rates and slow growth [40,[90][91][92][93][94][95][96][97][98]. These experimental results lay the foundation for the subsequent acquisition of the hybrid "yellow catfish Huangyou No.…”