This
paper presents a high-throughout method for simultaneous determination
of acetic acid and furfural in hydrothermal hydrolysate of lignocellulose
using a full evaporation headspace gas chromatographic technique.
In this method, the sample was acidified to pH 1.0–3.5 to a
complete acetic acid release from the medium, which also avoids the
risk for an acidized furfural decomposition at an elevated temperature.
With a maximum sampling volume of 5 μL, full evaporation for
both acetic acid and furfural at the headspace equilibration temperature
(100 °C) can be achieved in 3 min. The results showed that the
present method has a good precision [relative standard deviation (RSD)
< 4.67%] and accuracy (apparent recoveries of 95.0–104.8%
for acetic acid and 95.4–104.3% for furfural. The limits of
quantitation in the acetic acid and furfural detection were 0.339
and 0.208 μg, respectively. The present method is simple, rapid,
and accurate, which is suitable for rapid analysis of acetic acid
and furfural in lignocellulosic hydrolysate for research of biotechnology
screening and biofuel production.
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