To isolate high-quality total RNA from Fallopia multiflora tuberous roots is difficult because of the presence of high levels of carbohydrates, phenolics, and other secondary metabolites. Since several procedures specialized for RNA isolation from polysaccharides and phenols rich tissues have resulted in poor yields, in this study, we developed a modified protocol that was derived from the traditional CTAB method. The protocol was able to produce high-quality and intact RNA from the tuberous roots of F. multiflora. The yield of total RNA was more than 0.15 mg/g fresh weight, with an A260/A280 ratio of 1.9-2.0. The obtained RNA was of sufficient quality and suitable for downstream application such as reverse-transcription polymerase chain reaction (RT-PCR), Northern hybridization, and cDNA library construction. The protocol may also have wider applicability for total RNA isolation from other plant species with tuberous roots.
In the title compound, C9H7NO3S, the benzoisothiazolone ring system is essentially planar, with a maximum deviation of 0.013 (2) Å. In the crystal, molecules are linked via O—H⋯O hydrogen bonds, forming chains along [010]. In addition, weak intermolecular C—H⋯O hydrogen bonds are present.
The molecule of the title compound, C12H12O2, is essentially planar, with a maximum deviation from the mean plane of all non-H atoms of 0.038 (1) Å for the methyl C atom in the 8-position. The crystal structure is characterized by antiparallel π–π stacking along the c axis, with centroid–centroid distances as short as 3.866 (1) Å. In the crystal, C—H⋯O hydrogen bonds connect the molecules across the stacks into ribbons in the a-axis direction.
Activated carbon was prepared with sewage sludge as raw material and ZnCl2/CuCl2 as activating agent by low tempertaure carbonization method. The optimum preparation conditions were activation temperature 534 °C, activation time 60 min, ZnCl2 concentration 3 mol/L, CuCl2 concentration 0.3 mol/L, the iodine adsorportion value was 534 mg/g. The prepared sludge carbons have a large amount of micropores, also have mesopores and macropores, with a BET surface area at 784.89 m 2 /g and a Langmuir surface area at 1053.69 m 2 /g. Adsorption of pharmaceutical wastewater on sludge activated carbon was conducted. The data were fitted by Freundlich equation. The sludge activated carbon had fractal characteristics, the iodine adsorportion value grow with the fractal dimension and the roughness.
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