Group A rotaviruses of the family Reoviridae is one of the important intestinal pathogens causing diarrhea in piglets and humans. A human-porcine reassortment rotavirus, GDJM1, was identified from outbreak of diarrhea in suckling piglets and it associated with 60.00% (324/540) morbidity and 20.99% (68/324) mortality in Guangdong Province of China in 2022. Thus, to further characterize the evolutionary diversity of GDJM1, all gene segments were analyzed. The genome constellation was G9-P[19]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Nucleotide sequence identity and phylogenetic analyses showed that the VP6, VP7, NSP4 and NSP5 genes of GDJM1 were the most closely related to the respective genes of porcine strains, with the highest homology ranging from 95.65–98.55% identity. The remaining seven genes (VP1-VP4, NSP1-NSP3) were the most closely related to human strains, with the highest homology ranging from 91.83–96.69% similarity. Therefore, it is likely that GDJM1 emerged as the result of genetic reassortment between porcine and human rotaviruses. To our knowledge, this is the first report that a human-porcine reassortment G9P[19] RVA strain has been identified in mainland China, which providing important insights into evolutionary characterization of G9P[19] RVA strain, and reveals that the strain has a potential risk of cross-species transmission.

Porcine reproductive and respiratory syndrome (PRRS) is an economically important disease impacting the global pig industry, and it is characterized by reproductive disorder in sows and respiratory disorder in pigs of all ages. The PRRSV E protein is a nonglycosylated structural protein encoded by the ORF2b gene. The E protein is not necessary for the assembly of virus particles, but deletion of the E protein leads to transmissible virus particles not being produced. To better understand the structure and function of the E protein, we reviewed its genetic and evolutionary analysis, characteristics, subcellular localization and topology, ion channel activity, cellular immune response, additional biological functions, interactions with host proteins, interactions with PRRSV proteins, roles in infection, pathogenicity, and drugs. Therefore, this review can provide a theoretical basis for gaining an in-depth understanding of the E protein of PRRSV-2.