Muscle differentiation is an essential link in animal growth and development, and microRNA and mRNA are indispensable in skeletal muscle differentiation. To improve the meat quality and production of the Leizhou goat, it is vital to understand the molecular mechanism by which its skeletal muscle differentiates. By RNA sequencing (RNA-SEQ), we established miRNA-mRNA profiles of Leizhou goats at three stages: fetal day 70, 90, and 120. There were 991 differently expressed mRNAs and 39 differentially expressed miRNAs found, with the differentially expressed mRNAs mainly enriched in calcium ion binding, ECM-receptor interaction, and Focal adhesion. CKM and MYH3, two muscle differentiation markers, were significantly differentially expressed during this period. In addition, we found that chi-miR-129-5p, chi-miR-433, and chi-miR-24-3p co-regulate muscle differentiation with their target genes. Finally, we can confirm that muscle differentiation occurred in Leizhou goat between 90 and 120 days of the fetus. This study is helpful to better explore the molecular mechanism of goat muscle differentiation.
Meat yield and quality are important economic traits of livestock. Herein, longissimus dorsi (LD) muscles of Leizhou black goats aged 0, 3, and 6 months were used to identify differentially expressed messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) by high-throughput RNA sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to analyze differentially expressed genes. Expression levels of regulator of calcineurin 1 (RCAN1) and olfactory receptor 2AP1 (OR2AP1) were significantly different in LD muscles of goats aged 0, 3, and 6 months, indicating potentially important roles in postnatal muscle development. Differentially expressed lncRNAs and mRNAs were mainly enriched in biological processes and pathways related to cellular energy metabolism, consistent with previous studies. Three lncRNAs, TCONS_00074191, TCONS_00074190, and TCONS_00078361, may play a cis-acting role with methyltransferase-like 11B (METTL11B) genes and participate in the methylation of goat muscle proteins. Some of the identified genes may provide valuable resources for future studies on postnatal meat development in goat muscles.
Background: Meat yield and quality is one of the most important economic traits of livestock. Long non-coding RNAs (lncRNAs) is involved in the biological process of muscle differentiation and development. However, few studies have explored the regulatory role of lncRNA on muscle development after birth.Results: The longissimus dorsi muscle of Leizhou black goat aged 0, 3 and 6 months was used to identify differential mRNA and differential lncRNA by high-throughput RNA sequencing. GO, KEGG and interaction network were used to analyzed the differential genes. We found that some target genes of DE-mRNA and DE-lncRNA were mainly involved CN-NFAT signal pathway and the formation of branch fibers, which is related to normal muscle development. ENSCHIG00000006085 and ENSCHIG00000023270 are significantly concentrated in the biological process related to urea transporters, which has an important effect on maintaining the normal development of goats. ENSCHIG00000006085 and ENSCHIG00000023270 may be involved in the degradation of intracellular proteins and METTL11B may play a role in methylation modification of muscle proteins.Conclusions: Our results show that some lncRNAs are involved in the regulation of protein during goat muscle development as well as provide a valuable resource for lncRNA studies and make a deeper understanding of the genetic basis and molecular mechanisms of the development of goat skeletal muscle.
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