Identification of Key Functional Genes and LncRNAs Influencing Muscle Growth and Development in Leizhou Black Goats

Abstract

Meat yield and quality are important economic traits of livestock. Herein, longissimus dorsi (LD) muscles of Leizhou black goats aged 0, 3, and 6 months were used to identify differentially expressed messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) by high-throughput RNA sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to analyze differentially expressed genes. Expression levels of regulator of calcineurin 1 (RCAN1) and olfactory receptor 2AP1 (OR2AP1) were significantly different in LD muscles of goats aged 0, 3, and 6 months, indicating potentially important roles in postnatal muscle development. Differentially expressed lncRNAs and mRNAs were mainly enriched in biological processes and pathways related to cellular energy metabolism, consistent with previous studies. Three lncRNAs, TCONS_00074191, TCONS_00074190, and TCONS_00078361, may play a cis-acting role with methyltransferase-like 11B (METTL11B) genes and participate in the methylation of goat muscle proteins. Some of the identified genes may provide valuable resources for future studies on postnatal meat development in goat muscles.

Temporal Transcriptome Dynamics of Longissimus dorsi Reveals the Mechanism of the Differences in Muscle Development and IMF Deposition between Fuqing Goats and Nubian Goats

“…Skeletal muscle requires a great deal of energy and proteins to develop [ 50 ]. It has been reported that skeletal muscle hypertrophy requires an increase in the rate of protein synthesis, and one way in which this can be achieved is by increasing the translational capacity of muscle through ribosome biogenesis [ 51 ].…”

“…Furthermore, the categories "translation, ribosomal structure and biogenesis (13~27.66%)" and "energy production and conversion (12~25.53%)" were highly enriched according to COG classifications, which included the 154 highly expressed DEGs (FPKM > 10) of F0 vs. N0 (Figure 17B). Skeletal muscle requires a great deal of energy and proteins to develop [50]. It has been reported that skeletal muscle hypertrophy requires an increase in the rate of protein synthesis, and one way in which this can be achieved is by increasing the translational capacity of muscle through ribosome biogenesis [51].…”

Development of myofibers and muscle transcriptomic analysis in growing Yili geese

“…In recent years, numerous RNA-seq studies have investigated the growth performance of livestock and poultry. These studies aim to uncover the molecular mechanisms that regulate their growth traits ( Qian et al, 2023 ; Zhang et al, 2023 ; Zhao et al, 2023 ). Hu et al (2022) found that short photoperiod significantly promoted muscle development and significantly improved the redness of goose muscle, and identified the possible involvement of glycolysis/gluconeogenesis, calcium signaling pathway, and PI3K-Akt signaling pathway in the regulation of muscle development and meat color in geese by light through RNA-seq analysis.…”