Protein immobilization is particularly significant in proteomics, interactomics, and in vitro drug screening. It is an essential primary step for numerous biological techniques that rely on immobilized proteins with controlled orientation, high conformational stability, and high activity (CHH). These have challenged the current immobilization strategy and demanded increasing efforts for an efficient method to meet the CHH immobilization in a single step. Herein, we proposed a covalent inhibitorbased, one-step method for G protein-coupled receptor (GPCR) immobilization inspired by the covalent reaction between an epidermal growth factor receptor (EGFR)-tag and its inhibitor ibrutinib. We immobilized endothelin receptor A (ETA) containing a fusion EGFR tag onto an ibrutinib-coated macroporous silica gel. The immobilized ETA proved to have demonstrable ligand-binding activity and specificity, thus resulting in a chromatographic technology allowing receptor−ligand interaction analysis and lead identification. Such immobilization method is attractable, owing to the properties of mild reacting conditions, fast rate, high yield, and good stability of the conjugated protein. It will be applicable to biochips, biosensors, and biocatalysts.
The development of environmentally
friendly and sustainable corrosion
protection technologies is a longstanding yet difficult problem, especially
for the marine environment. The utilization of living biofilms isolated
from local environments is an effective strategy for infrastructure
protection. In this study, three aerobic marine bacteria, Tenacibaculum mesophilum D-6, Tenacibaculum
litoreum W-4, and Bacillus sp. Y-6, with strong biofilm-forming abilities were isolated and
evaluated for the corrosion protection of X80 carbon steel. The corrosion
inhibitory effect of the bacteria was found to be closely related
to their biofilm-forming abilities. This conclusion was corroborated
by biofilm characterization, electrochemical tests, weight loss analysis,
and corrosion product analysis. Moreover, secreted extracellular polymeric
substances were identified to play significant roles in corrosion
inhibition. Herein, we proposed a novel, eco-friendly, and cost-effective
method for corrosion protection of carbon steels in the marine environment,
providing guiding principles for identifying corrosion inhibitory
bacteria from the local marine environment.
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