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The object of this paper is a permanent magnet synchronous linear motor (PMLSM), whose control method is based on a model-referenced adaptive system (MRAS), and it analyses the speed identification of a permanent magnet synchronous linear motor without position sensors. The article proposes a new model-referenced adaptive method, which utilises a sliding-mode variable structure control method (SMC), to replace the PI control algorithm utilised in conventional model-referenced adaptive algorithm. The control system of the PMLSM is therefore designed and studied based on the change of the adaptive law in model-referenced adaption. The mathematical model of the PMLSM itself is chosen as the reference model, and the feedback magnetic chain model of the motor output is chosen as the adjustable model, replacing the conventional current model and simplifying the control algorithm. The sliding mode surface of the sliding mode variable structure control algorithm is constructed using the reference model and the output error of the adjustable model. Through theoretical analysis and simulation models built by MATLAB/Simulink simulation software, the simulation results show that the designed PMLSM speed induction-free control system MRAS speed observer based on the sliding mode variable structure has strong robustness and excellent dynamic static performance. The advantages verified by the new algorithm achieve the experimental purpose of the expected assumptions.

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The aim of the study was to investigate whether the negative effects of dietary glycinin are linked to the structural integrity damage, apoptosis promotion and microbiota alteration in the intestine of orange-spotted grouper (Epinephelus coioides). The basal diet (FM diet) was formulated to contain 48% protein and 11% lipid. Fish meal was replaced by soybean meal (SBM) in FM diets to prepare the SBM diet. Two experimental diets were prepared, containing 4.5% and 10% glycinin in the FM diets (G-4.5 and G-10, respectively). Triplicate groups of 20 fish in each tank (initial weight: 8.01 ± 0.10 g) were fed the four diets across an 8 week growth trial period. Fish fed SBM diets had reduced growth rate, hepatosomatic index, liver total antioxidant capacity and GSH-Px activity, but elevated liver MDA content vs. FM diets. The G-4.5 exhibited maximum growth and the G-10 exhibited a comparable growth with that of the FM diet group. The SBM and G-10 diets down-regulated intestinal tight junction function genes (occludin, claudin-3 and ZO-1) and intestinal apoptosis genes (caspase-3, caspase-8, caspase-9, bcl-2 and bcl-xL), but elevated blood diamine oxidase activity, D-lactic acid and endotoxin contents related to intestinal mucosal permeability, as well as the number of intestinal apoptosis vs FM diets. The intestinal abundance of phylum Proteobacteria and genus Vibrio in SBM diets were higher than those in groups receiving other diets. As for the expression of intestinal inflammatory factor genes, in SBM and G-10 diets vs. FM diets, pro-inflammatory genes (TNF-α, IL-1β and IL-8) were up-regulated, but anti-inflammatory genes (TGF-β1 and IL-10) were down-regulated. The results indicate that dietary 10% glycinin rather than 4.5% glycinin could decrease hepatic antioxidant ability and destroy both the intestinal microbiota profile and morphological integrity through disrupting the tight junction structure of the intestine, increasing intestinal mucosal permeability and apoptosis. These results further trigger intestinal inflammatory reactions and even enteritis, ultimately leading to the poor growth of fish.