Optimization of the
route to the sap-feeding insecticidal candidate
tyclopyrazoflor featuring [3 + 2] cyclization of 3-hydrazinopyridine·2HCl
and methyl acrylate is described. The key impurities in the [3 + 2]
cyclization were identified and successfully controlled after optimization.
The hazards associated with oxidation of an intermediate pyrazolidin-3-one
using the incompatible combination of potassium persulfate and N,N-dimethylformamide (DMF) were avoided
by using potassium ferricyanide in the presence of potassium hydroxide
in water. The two elimination impurities in the ethylation step to
produce tyclopyrazoflor were successfully minimized using ethyl iodide
in the presence of cesium carbonate in DMF at 0 °C. The overall
yield for this seven-step synthesis of tyclopyrazoflor was improved
from 10% to 41% after the optimization detailed herein.
Starch digestion in the small intestine in ruminants is relatively lower compared with that in monogastric animals, likely due to low pancreatic α-amylase secretion. Previous studies suggested that leucine could increase pancreatic α-amylase secretion in the small intestine of heifers cannulated with abomasal, duodenal, and ileal catheters. However, the surgical procedures probably have an effect on pancreatic function. Thus, we used rumen-protected leucine (RP-Leu) to explore its effect on small intestinal digestion of starch in calves without any surgery in 3 experiments. The first experiment was to explore whether RP-Leu could improve post-ruminal starch digestion in 5-mo-old calves (158 ± 19 kg body weight ± standard deviation). We found that RP-Leu did not affect rumen fermentation profile or wholetract starch digestibility, but it increased blood glucose concentration and fecal pH and decreased fecal propionate molar proportion. Additionally, RP-Leu increased fibrolytic genera Ruminiclostridium and Pseudobutyrivibrio and decreased the amylolytic genus of Faecalibacterium. The second experiment compared RP-Leu and rumen-protected lysine (RP-Lys) for their effects on post-ruminal starch digestion in 6-mo-old calves (201 ± 24 kg body weight). The responses of blood glucose concentration, fecal pH, fecal propionate proportion, and starch digestibility to RP-Leu supplementation were similar to those observed in experiment 1. Cellulolytic family Ruminococcaceae and Bacteroidales BS11 gut group tended to be increased by RP-Leu. In contrast, RP-Lys showed no significant influence on the above measurements. The third experiment determined the interaction between RP-Leu and rumen-escape starch (RES) on the small intestinal digestion of starch in 8-mo-old calves (289 ± 26 kg body weight). An interaction between RP-Leu and RES levels was observed in fecal butyrate concentration and the relative abundance of family Bacteroidaceae, and genera Ruminococcaceae UCG-005 and Bacteroides. We found that RP-Leu tended to increase the abundance of fecal Firmicutes and decrease Spirochaetae. In conclusion, RP-Leu, but not RP-Lys, increased blood glucose concentration and decreased the amount of starch fermented in the hindgut in a RES dose-dependent manner, suggesting that RP-Leu might stimulate starch digestion in the small intestine.
Phenolic compounds have very strong toxicity, so it has been paid sharply attention to find an effective way of controlling the wastewater containing phenolic compounds. The work on this subject done by domestic and overseas scholars is studied in this paper, and the progress of researches on low-temperature plasma treatment is summarized through the electrical discharge types, mechanism, kinetics of phenolic compounds decomposition and combination of several methods with low-temperature plasma treatment. In addition, the crucial problem and the developing tendency on low-temperature plasma treatment for phenol-bearing wastewater are briefly discussed
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