Ulvans, complex polysaccharides found in the ulvales (green seaweed) cell wall, contain predominantly 3-sulfated rhamnose (Rha3S) linked to either d-glucuronic acid, l-iduronic acid or d-xylose.

No abstract

A geographic information system (GIS)-based analysis tool, the Taihu Basin Risk Assessment System (TBRAS), is developed to perform scenario analysis of future flood risk changes in the Taihu Basin. The TBRAS integrates modules of climate modelling, hydrological and hydraulic modelling, dyke reliability analysis, socioeconomic analysis, and damage assessment. The results indicate that the TBRAS can provide a broad-scale simulation of the flooding processes and damage evaluation for the entire Taihu Basin. Future climate change by 2050 in precipitation alone can approximately result in four or five times of risk increase compared with the 2005 baseline risk. Socioeconomic changes alone can increase flood risk by four to six times. The paper reveals that, without any proactive responses, the combination of socioeconomic change, sea-level rise, and other climate changes can substantially increase the overall flood risks to 15-37 times of the present situation. We therefore conclude that both structural and non-structural measures for reducing flood risks are required and provide a set of response options for managing and adapting future flood risk changes. the Taihu Basin Risk Assessment System (TBRAS), is developed to carry out such quantitative risk analysis. Other papers (Harvey et al., 2009, Yu et al., 2012, and the other bs_bs_banner J Flood Risk Management 6 (2013) 57-68

No abstract

No abstract

BackgroundBiosynthesis of steroidal drugs is of great benefit in pharmaceutical manufacturing as the process involves efficient enzymatic catalysis at ambient temperature and atmospheric pressure compared to chemical synthesis. 3-ketosteroid-∆1-dehydrogenase from Arthrobacter simplex (KsdD3) catalyzes 1,2-desaturation of steroidal substrates with FAD as a cofactor.ResultsRecombinant KsdD3 exhibited organic solvent tolerance. W117, F296, W299, et al., which were located in substrate-binding cavity, were predicted to form hydrophobic interaction with the substrate. Structure-based site-directed saturation mutagenesis of KsdD3 was performed with W299 mutants, which resulted in improved catalytic activities toward various steroidal substrates. W299A showed the highest increase in catalytic efficiency (kcat/Km) compared with the wild-type enzyme. Homology modelling revealed that the mutants enlarged the active site cavity and relieved the steric interference facilitating recognition of C17 hydroxyl/carbonyl steroidal substrates. Steered molecular dynamics simulations revealed that W299A/G decreased the potential energy barrier of association of substrates and dissociation of the corresponding products. The biotransformation of AD with enzymatic catalysis and resting cells harbouring KsdD3 WT/mutants revealed that W299A catalyzed the maximum ADD yields of 71 and 95% by enzymatic catalysis and resting cell conversion respectively, compared with the wild type (38 and 75%, respectively).ConclusionsThe successful rational design of functional KsdD3 greatly advanced our understanding of KsdD family enzymes. Structure-based site-directed saturation mutagenesis and biochemical data were used to design KsdD3 mutants with a higher catalytic activity and broader selectivity. Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0981-0) contains supplementary material, which is available to authorized users.

This paper presents the results of a qualitative analysis of future flood risk in the Taihu Basin, China, performed using an adaptation of the UK Foresight Future Flooding approach. Drivers of increased flood risk were identified and ranked according to their importance in contributing to future flooding by experts and stakeholders working within an inclusive, participatory framework. Management responses to increasing flood risk were also identified and assessed in terms, first, of their potential to reduce flood risks and, second, their sustainability. This analysis provides the foundation for quantitative flood risk modelling to be performed in the next phase of the project. It has also added value to flood risk management in the Taihu Basin by bringing stakeholders together to develop a shared understanding of the flooding system and the relative importance of multiple flood risk drivers and responses. Together, the qualitative and quantitative analyses will provide a comprehensive vision of possible future flood risk to inform policy development and decision making.

The Taihu Basin, situated on the south side of the Yangtze delta, is a large flood-prone area that has urbanised rapidly. The risk of flooding is set to increase in future because of continued economic development and the impacts of climate change. Scenario analysis has been adopted to help understand the potential impacts of long-term change on flood risk, including the effects of climate change and socio-economic development, thus providing the basis for the development of sustainable flood risk management strategies. This paper describes the flooding processes in the Taihu Basin and sets out strategic questions that the scenario analysis sought to address. It provides an overview of the assessment framework adopted in the China/UK scientific cooperation project 'China-UK Scenario Analysis Technology for River Basin Flood Risk Management in the Taihu Basin' . Further details of the component studies carried out as part of the project are given in the associated papers in this special issue. Hangzhou Bay and eastward to the East Sea through a system of major high-level carriers and the internal river network. bs_bs_banner J Flood Risk Management 6 (2013) 3-13