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A new kind of acid sensitive tetrahydrofuranyl (THF) linker was synthesized and then reacted with 5-(6)-carboxytetramethylrhodaminesuccinimidyl ester (5(6)-TAMRA, SE), followed by di(N-succinimidyl) carbonate (DSC) and modified 2'-deoxyuridine triphosphate (dUTP); the final product, as a reversible terminator for DNA sequencing by synthesis (DNA SBS), was given obtained and confirmed by 1H-NMR, 31P-NMR, and HRMS with purity of up to 99%. The synthesized dye-labeled terminator incorporated into DNA strand successfully, and the fluorophore was cleaved completely under acidic conditions. The preliminary results encourage us to explore more acid-sensitive linkers for DNA SBS to increase the cleavage efficiency under weakly acidic conditions.

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This paper deals with a critical study of Coordinated Management of Meaning Theory (CMM) as a practical theory and its function to employ a systematic way of conversation in a critical speech. The present study is based on a brief introduction and background perspective of the theory along with its different stages in relation with to address the issues of social conversations. The researcher also has included opinions of many other practitioners of CMM theory and their appreciations, suggestions, and concerns are discussed to make the understanding of the theory in a more clear and comprehensive way. The study concludes that CMM has influenced interpersonal and intercultural communication, management of communication, social communication, conflict, and mediation in communication and last but not the least consultancy and therapy through communication.

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