In fermentation process, substrate concentration control is important in increasing product concentration, productivity and substrate yield so as to decrease the production cost. But, the feedback control of substrate concentration is difficult for the lack of sensor for online measurement of substrate concentration. In order to overcome above problem, dissolved oxygen (DO) stat strategy was developed for feedback control of glucose feeding in fedbatch fermentation using the abrupt increase of DO as the indicator for glucose depletion and glucose feeding. In this research, the DO stat control strategy for bioreactor control will be developed and the control process will be simulated. The simulation results indicate that the DO stat strategy is effective in glucose feeding control.
Bioprocess automation is developing fast for the reasons of quality control, production cost reduction et. al.. Bioreactor is the central equipment of bioprocess. Construction of computer controlled bioreactor is need for bioprocess automation. In this research, a two level hierarchical structure computer control bioreactor system is designed and constructed. The lower level plays the major role of set-point feedback control of process variables and the higher level plays the major role of graphical human interface and biological mathematical model solution. This control system has advantages of high reliability and flexibility than the single level digital control system, and has plenty of functions and high practicality compared with a normal commercial one.
Fermentation process using recombinant strain for production of recombinant protein is widely used in commercialization of the biotechnologies. The continuous stirred tank reactor (CSTR) is a typical microbial cultivation method, has the major advantage of high productivity. Mathematical modeling and simulation is useful for analysis and optimization of the CSTR fermentation process. Most of the mathematical models developed for CSTR are black box models without information of the intracellular dynamics and regulations. In this research, a mathematical model is built based on gene regulation for recombinant protein production using CSTR, and simulation is made using this model.
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