Bioreactor Substrate Feeding Control Using DO Stat Control Strategy: A Modeling and Computer Simulation Study

Abstract

In fermentation process, substrate concentration control is important in increasing product concentration, productivity and substrate yield so as to decrease the production cost. But, the feedback control of substrate concentration is difficult for the lack of sensor for online measurement of substrate concentration. In order to overcome above problem, dissolved oxygen (DO) stat strategy was developed for feedback control of glucose feeding in fedbatch fermentation using the abrupt increase of DO as the indicator for glucose depletion and glucose feeding. In this research, the DO stat control strategy for bioreactor control will be developed and the control process will be simulated. The simulation results indicate that the DO stat strategy is effective in glucose feeding control.

Computer Simulation of Bioprocess

“…Then, the control system will monitor the next sudden DO rise for glucose feeding control, which strategy can maintain glucose concentration in low level and is called DO stat control strategy. This control strategy has been analyzed by modeling method [12]. …”

“…In order to simulate the "bell" type specific growth rate curve and the "S" type cell growth curve more accurately, the following model is developed shown by Eqs. (12) and (13) [5]…”