The COVID-19 pandemic brings unprecedented crisis for public health and economics in the world. Detecting specific antibodies to SARS-CoV-2 is a powerful supplement for the diagnosis of COVID-19 and is important for epidemiological studies and vaccine validations. Herein, a rapid and quantitative detection method of anti-SARS-CoV-2 IgG antibody was built based on the optofluidic point-of-care testing fluorescence biosensor. Without complicated steps needed, the portable system is suitable for on-site sensitive determination of anti-SARS-CoV-2 IgG antibody in serum. Under the optimal conditions, the whole detection procedure is about 25 min with a detection limit of 12.5 ng/mL that can well meet the diagnostic requirements. The method was not obviously affected by IgM and serum matrix and demonstrated to have good stability and reliability in real sample analysis. Compared to ELISA test results, the proposed method exhibits several advantages including wider measurement range and easier operation. The method provides a universal platform for rapid and quantitative analysis of other related biomarkers, which is of significance for the prevention and control of COVID-19 pandemic.

No abstract

No abstract

A novel, compact, cost-effective, and robust label-free all-fiber optofluidic biosensor (LF-AOB) based on Fresnel reflection mechanism was built through integrating single-multi mode fiber coupler and highly sensitive micro-photodetector. The Fresnel reflection light intensity detected by the LF-AOB greatly depended on the RI change on the end-surface of the fiber probe according to experimental and simulation results. The capability of the LF-AOB for real-time in situ detection in optofluidic system were verified by measuring salt and protein solution, and the lowest limit of detection was 1.0 × 10 −6 RIU. Our proposed theory can effectively eliminate the influence of light intensity fluctuation, and one-point calibration method of sensor performance is conducive for rapid and convenient detection of targets. Label-free sensitive detection of SARS-Cov-2 Spike protein receptor-binding domain (S-RBD) and the binding kinetics assay between S-RBD and anti-S-RBD antibody were achieved using the LF-AOB. These contributed to the elegant design of all-fiber optical system with high efficiency, high resolution and sensitivity of micro-photodetector, and enhanced interaction between the light and the samples at the liquid-sensor interface because of the large surface area of the multi-mode fiber probe. The LF-AOB can be extended as a universal sensing platform to measure other factors associated with refractive index because its high sensitivity, low sample consumption (∼160 nL), and capability of real-time in situ detection.

No abstract

This paper develops an improved portable and reusable evanescent wave optofluidic immunosensor (OIP-v2) for rapid and sensitive on-site determination of deoxynivalenol (DON), one of the most frequently detected mycotoxins mainly produced by Fusarium species. Using the bifunctional reagent N,N′-Disuccinimidyl carbonate, deoxynivalenol-bovine-serum-albumin (DON-BSA) were covalently modified onto a bio-probe surface as biorecognition elements, whose robustness allowed it to perform multiple detections without significant activity loss. An indirect competitive immunoassay strategy was applied for DON detection. Under optimal conditions, the limit of detection of 0.11 μg/L and the linear dynamic detection range of 0.43 to 36.61 μg/L was obtained when the concentration of the Cy5.5-anti-DON antibody was 0.25 μg/mL. The OIP-v2 was also applied to detect DON in various cereals, and the recoveries ranged from 81% to 127%. The correlation between OIP-v2 and enzyme-linked immunosorbent assay (ELISA) through the simultaneous detection of maize-positive samples was in good agreement (R2 = 0.9891).

No abstract

No abstract