[1] Global high-resolution (3-hourly, 0.1°Â 0.1°longitude-latitude) water vapor (6.7 mm) and window (11 mm) radiances from multiple geostationary satellites are used to document the diurnal cycle of upper tropospheric relative humidity (UTH) and its relationship to deep convection and high clouds in the whole tropics and to evaluate the ability of the new Geophysical Fluid Dynamics Laboratory (GFDL) global atmosphere and land model (AM2/LM2) to simulate these diurnal variations. Similar to the diurnal cycle of deep convection and high clouds, coherent diurnal variations in UTH are also observed over the deep convective regions, where the daily mean UTH is high. In addition, the diurnal cycle in UTH also features a land-sea contrast: stronger over land but weaker over ocean. UTH tends to peak around midnight over ocean in contrast to 0300 LST over land. Furthermore, UTH is observed to lag high cloud cover by $6 hours, and the latter further lags deep convection, implying that deep convection serves to moisten the upper troposphere through the evaporation of the cirrus anvil clouds generated by deep convection. Compared to the satellite observations, AM2/LM2 can roughly capture the diurnal phases of deep convection, high cloud cover, and UTH over land; however, the magnitudes are noticeably weaker in the model. Over the oceans the AM2/LM2 has difficulty in simulating both the diurnal phase and amplitude of these quantities. These results reveal some important deficiencies in the model's convection and cloud parameterization schemes and suggest the lack of a diurnal cycle in SST may be a shortcoming in the boundary forcing for atmospheric models.

Histone methylation plays an important role in eukaryotic transcriptional regulation. A number of histone methyltransferases (HMTases) with distinct functions have been identified. The HSPC069/HYPB gene was originally isolated from the human hematopoietic stem/progenitor cells (HSPCs), and it was also identified as a huntingtin interacting protein, implicated in the pathogenesis of Huntington disease (HD). However, its biochemical function is poorly understood. Here we report the structural and functional characterization of the huntingtin interacting protein B (HYPB). 1) The triplicate AWS-SET-PostSET domains mediate a histone H3 lysine 36 specific HMTase activity. 2) A low charged region that is rich in glutamine and proline has been characterized as a novel transcriptional activation domain. The structural features of this region are evolutionarily conserved in vertebrates. 3) Coimmunoprecipitation assays indicate that HYPB protein associates with hyperphosphorylated RNA polymerase II (RNAPII) but not the unphosphorylated form. Furthermore, the RNAPII-association region of HYPB protein has been identified to encompass the C-terminal 142 amino acids. Thus, our results suggest that HYPB HMTase may coordinate histone methylation and transcriptional regulation in mammals and open perspective for the further study of the potential roles of HYPB protein in hematopoiesis and pathogenesis of HD.Nucleosome, the bead-like unit of DNA packaging in eukaryotic cells, consists of DNA wound around a protein core made up of eight histone molecules. Covalent modifications of the N-terminal tails of the core histones have emerged as key regulatory mechanisms of gene expression (1-3). These histone modifications, including acetylation, phosphorylation, ubiquitination, and methylation, create both synergistic and antagonistic signals that correlate with the transcriptional activity of a gene, through recruiting/dispelling some protein complexes or through changing the structure of chromatin to allow access for RNA polymerase to initiate transcription. Moreover, these histone modifications and the consequent changes in chromatin structure may serve as an epigenetic marking system that is responsible for establishing and maintaining the heritable programs of gene expression during cellular differentiation and organism development (4, 5).Methylations of histone lysine residues, with exception of H3 lysine 79, are catalyzed by a family of SET domain-containing proteins (6). The SET domain is an evolutionarily conserved, ϳ130-amino acid sequence motif. It was originally identified in members of polycomb group (PcG), trithorax group (trxG), and Su(var) genes and was named after the genes Su(var)3-9, Enhancer of zeste (E(z)) and trithorax (trx) (7). Not all SET domain-containing proteins possess histone methyltransferase (HMTase) 5 activities. The cysteine-rich regions adjacent to the SET domains are also required (8,9). In addition to the SET domains, most HMTases carry other functional domains such as transcriptional activation or re...

The emissivity model for rough sea surface [Remote Sensing Environ. 24, 313-329 (1988)] is inspected in light of the measured surface emissivity. In the presence of moderate wind (5 m/s or less), the emissivity model is found to be adequate for small to moderate view angles. For large view angles, the discrepancy between the computed and the measured emissivity is large, but one can reduce this considerably by incorporating the reflected sea surface emission into the emissivity model. In addition, examination of the spectral variation of the observed and computed emissivity suggests the need for refined measurements of the complex refractive index. An improved model is constructed to calculate the rough sea surface emissivity that can be used to provide accurate estimates of sea surface skin temperatures from remotely sensed radiometric measurements. An important feature of the improved model is that the computed sea surface emissivity is only weakly dependent on wind speed for most view angles used in practice.

Three hundred cDNAs containing putatively entire open reading frames (ORFs) for previously undefined genes were obtained from CD34+ hematopoietic stem/progenitor cells (HSPCs), based on EST cataloging, clone sequencing, in silico cloning, and rapid amplification of cDNA ends (RACE). The cDNA sizes ranged from 360 to 3496 bp and their ORFs coded for peptides of 58-752 amino acids. Public database search indicated that 225 cDNAs exhibited sequence similarities to genes identified across a variety of species. Homology analysis led to the recognition of 50 basic structural motifs/domains among these cDNAs. Genomic exon-intron organization could be established in 243 genes by integration of cDNA data with genome sequence information. Interestingly, a new gene named as HSPC070 on 3p was found to share a sequence of 105bp in 3Ј UTR with RAF gene in reversed transcription orientation. Chromosomal localizations were obtained using electronic mapping for 192 genes and with radiation hybrid (RH) for 38 genes. Macroarray technique was applied to screen the gene expression patterns in five hematopoietic cell lines (NB4, HL60, U937, K562, and Jurkat) and a number of genes with differential expression were found. The resource work has provided a wide range of information useful not only for expression genomics and annotation of genomic DNA sequence, but also for further research on the function of genes involved in hematopoietic development and differentiation.

Images captured under poor illumination conditions often exhibit characteristics such as low brightness, low contrast, a narrow gray range, and color distortion, as well as considerable noise, which seriously affect the subjective visual effect on human eyes and greatly limit the performance of various machine vision systems. The role of low-light image enhancement is to improve the visual effect of such images for the benefit of subsequent processing. This paper reviews the main techniques of low-light image enhancement developed over the past decades. First, we present a new classification of these algorithms, dividing them into seven categories: gray transformation methods, histogram equalization methods, Retinex methods, frequency-domain methods, image fusion methods, defogging model methods and machine learning methods. Then, all the categories of methods, including subcategories, are introduced in accordance with their principles and characteristics. In addition, various quality evaluation methods for enhanced images are detailed, and comparisons of different algorithms are discussed. Finally, the current research progress is summarized, and future research directions are suggested. INDEX TERMS Review, survey, low-light image enhancement, Retinex method, image enhancement, quality evaluation.

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A general alkylation of heterocycles using a simple palladium catalyst is reported. Most classes of heterocycles, including indoles and pyridines, efficiently coupled with unactivated secondary and tertiary alkyl halides. An alkyl radical addition to neutral heteroarenes is most likely involved.