, inventories, checklists of Malaysian algae, including ecological studies and applied phycology publications were produced. The advancement of gene technology encouraged the ABSTRAK Satu tinjauan keatas 30 tapak pesampelan di Semenanjung Malaysia, mewakili lima jenis habitat,iaitu kolam air panas, tasik air tawar yang mengalami eutrofikasi, kolam kumbahan kilang kelapa sawit, habitat air payau dan habitat air masin, menghasilkan 79 sampel persekitaran. Jumlah keseluruhan 73 taxa dan 52 genera alga dikenalpasti, dengan 9 genera dan 11 taxa Cyanophyta; 25 genera dan 33 taxa Baccilariophyta; 13 genera dan 16 taxa Chlorophyta dan 5 genera dan 13 taxa Euglenophyta. Dari segi bilangan sel, sampel daripada kilang kelapa sawit adalah paling tinggi bilangan selnya. Daripada sampel dipencilkan yang diperolehi daripada sampel yang dikumpulkan, hanya enam genera, iaitu Cyanophyta Cyanosarcina, Chlorophyta Chlorella, Chlamydomonas, Chlorococcum, Scenedesmus dan Euglenophyta Euglena akan dibincangkan dari segi pertumbuhannya dan profil biokimia didalam kertas kerja ini. Chlorococcum dan Euglena mempunyai kadar pertumbuhan spesifik, µ, paling tinggi diikuti oleh Chlorella dan Scenedesmus. Produktiviti biojisim pada hari ke-12 secara amnya adalah lebih tinggi daripada hari ke-8 untuk semua genera. Produktiviti biojisim paling tinggi adalah daripada Chlorella diikuti oleh Chlamydomonas. Produktiviti lemak paling tinggi adalah daripada Chlorella pada hari ke-12. Chlorella mempunyai produktiviti karbohidrat paling tinggi diikuti Chlamydomonas. Chlorella dan Chlamydomonas mempunyai produktiviti protein paling tinggi. Dalam tinjauan pendek ini, terdapat alga tertentu yang telah dikenalpasti dan dipencilkan yang dapat membuktikn ia mempunyai potensi untuk digunakan sebagai bahan asas utama untuk penghasillan bahan api biologi.
Background. Mechanical stress was investigated as a mean to harvest microalgal lipids without affecting algal cells’ viability. Monoraphidium minutum was cultivated in laboratory-scale photobioreactors and suspension cultures were submitted to mechanical stress to compare a cyclone, a centrifuge and a homogenizer. Lipid content within the extracellular medium was analyzed prior to and after treatment, and the amount of released lipids was quantified. Algal cell viability was also evaluated before and after treatment.Results. After mechanical-stress treatments, 7.0 to 12.7% of the intracellular lipids of Monoraphidium minutum were released and found in the extracellular medium, while recovered algal cells presented low levels of disruption after treatments.Conclusions. To the best of our knowledge, this is the first proof-of-concept demonstration on the use of mechanical stress for lipid extraction from viable microalgae. Certain level of centrifugation proved to make algae release around 10% of their lipids to the extracellular medium. This mostly exploratory work calls for deeper investigation, paving the way for a biofuel production based on continuous lipid recovery and microalgae reuses.
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