Feasibility of lipid mechanical extraction from viable Monoraphidium minutum

Dommange, Xavier; Tanguy, Philippe A.; Jolicœur, Mario

doi:10.1515/micbi-2015-0002

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…For instance, some protocols favor imposing a high mechanical stress such as ultrasound treatment [ 3 ], resulting in a high cell disruption efficiency level. For comparison, a low shear stress approach such as using a hydrocyclone only leads to ~10% cell lipids extraction efficiency but microalgae cells remain viable [ 31 ]. Overall, the solvents perform lipid extraction, which explains the amount of work dedicated to identify the most efficient solvents combination.…”

Section: Introductionmentioning

confidence: 99%

Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides

et al. 2017

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Background Microalgae have the potential to rapidly accumulate lipids of high interest for the food, cosmetics, pharmaceutical and energy (e.g. biodiesel) industries. However, current lipid extraction methods show efficiency limitation and until now, extraction protocols have not been fully optimized for specific lipid compounds. The present study thus presents a novel lipid extraction method, consisting in the addition of a water treatment of biomass between the two-stage solvent extraction steps of current extraction methods. The resulting modified method not only enhances lipid extraction efficiency, but also yields a higher triacylglycerols (TAG) ratio, which is highly desirable for biodiesel production.ResultsModification of four existing methods using acetone, chloroform/methanol (Chl/Met), chloroform/methanol/H2O (Chl/Met/H2O) and dichloromethane/methanol (Dic/Met) showed respective lipid extraction yield enhancement of 72.3, 35.8, 60.3 and 60.9%. The modified acetone method resulted in the highest extraction yield, with 68.9 ± 0.2% DW total lipids. Extraction of TAG was particularly improved with the water treatment, especially for the Chl/Met/H2O and Dic/Met methods. The acetone method with the water treatment led to the highest extraction level of TAG with 73.7 ± 7.3 µg/mg DW, which is 130.8 ± 10.6% higher than the maximum value obtained for the four classical methods (31.9 ± 4.6 µg/mg DW). Interestingly, the water treatment preferentially improved the extraction of intracellular fractions, i.e. TAG, sterols, and free fatty acids, compared to the lipid fractions of the cell membranes, which are constituted of phospholipids (PL), acetone mobile polar lipids and hydrocarbons. Finally, from the 32 fatty acids analyzed for both neutral lipids (NL) and polar lipids (PL) fractions, it is clear that the water treatment greatly improves NL-to-PL ratio for the four standard methods assessed.ConclusionWater treatment of biomass after the first solvent extraction step helps the subsequent release of intracellular lipids in the second extraction step, thus improving the global lipids extraction yield. In addition, the water treatment positively modifies the intracellular lipid class ratios of the final extract, in which TAG ratio is significantly increased without changes in the fatty acids composition. The novel method thus provides an efficient way to improve lipid extraction yield of existing methods, as well as selectively favoring TAG, a lipid of the upmost interest for biodiesel production.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0633-9) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides

et al. 2017

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“…There are also other algal species such as Tetradesmus sp ( T ) and Monoraphidium sp ( M ) that requires much attention in phycoremediation studies for the removal of antibiotics. Tetradesmus sp ( T ) and Monoraphidium sp ( M ) have shown good phycoremediation properties and have the potential to produce valuable biomass with good biochemical properties 24 – 27 , but both the species have yet not been tested for their potential in the removal of FQs. Phycoremediation potential can be stunned when the test values are greater than EC 50 .…”

Section: Introductionmentioning

confidence: 99%

An investigation on removal of ciprofloxacin and norfloxacin by phycoremediation with an emphasis on acute toxicity and biochemical composition

Ricky,

Shanthakumar

2023

Sci Rep

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Ciprofloxacin (CIP) and norfloxacin (NOR) belong to the class of emerging contaminants that are frequently detected in the aquatic environment as a binary mixture, responsible for the development of antibiotic-resistant genes and antibiotic-resistant bacteria. This study aims to investigate five different algal species Chlorella vulgaris (Cv), Chlorella pyrenoidosa (Cp), Scenedesmus obliquus (So), Tetradesmus sp (T) and Monoraphidium sp (M) for their tolerance and removal of binary mixture. The effects on biochemical composition in the algal species concerning the binary mixture and its removal efficiency are first reported in this study. The acute toxicity (96 h EC50) values are in the order of So > Cp > T > M > Cv, Chlorella vulgaris is the most sensitive algal species with 17.73 ± 0.24 mg/L and Scenedesmus obliquus is the least sensitive algal species with 39.19 ± 0.79 mg/L. The removal efficiency of the binary mixture was found to be in the order of So > Cp > T > M > Cv, Scenedesmus obliquus removed CIP (52.4%) and NOR (87.5%) with biodegradation as the major contributing removal mechanism. Furthermore, less toxic biotransformed products were detected in Scenedesmus obliquus and the biochemical characterization revealed that the growth-stimulating effect is higher with lipid (35%), carbohydrate (18%), and protein (33%) providing an advantage in the production of valuable biomass.

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Valorization of Monoraphidium sp. microalgal biomass for human nutrition applications

Georgiou,

Exarhopoulos,

Charisis

et al. 2024

J Appl Phycol

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The green microalga Monoraphidium sp. has potential for biodiesel production since it grows fast and can accumulate high levels of lipids. However, there is a lack of information on the potential use in human nutrition. In this work Monoraphidium sp. was characterized in terms of macronutrients with a special focus on the fatty acid profile of the lipid fraction and on the amino acid profile of the proteins. Furthermore, aiming at nutrient valorization, various methods for lipid extraction that could be used in the production of high quality and safe foods were investigated. To this end, the focus was on cell disruption methods in order to optimize oil recovery. The Monoraphidium sp. biomass had a high protein concentration (44.5 %) and a relatively low lipid concentration (12.5 %) but was rich in ω-3 fatty acids demonstrating its high nutritive value. Regarding cell disruption, ultrasonication and high-speed homogenization were insufficient to disrupt cells under the conditions examined. On the other hand, the effectiveness of the ball milling was regulated via processing time and water-to-biomass ratio and complete cell disruption could be achieved by this method. For lipid extraction, solvent extraction using a mixture of food-grade hexane and ethanol resulted in an oil recovery of 70.4 %, whereas using ethanol alone as an environmentally friendly solvent resulted in an oil recovery equal to 54 %. Supercritical CO2 extraction resulted in a lower oil recovery (25 %), whereas ethanol addition, as a cosolvent to CO2, significantly increased the oil recovery (60 %).

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Feasibility of lipid mechanical extraction from viable Monoraphidium minutum

Cited by 3 publications

References 30 publications

Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides

Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides

An investigation on removal of ciprofloxacin and norfloxacin by phycoremediation with an emphasis on acute toxicity and biochemical composition

Valorization of Monoraphidium sp. microalgal biomass for human nutrition applications

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