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Citation: DENG X.T., SHI, J.J. and KONG, M.G., 2007. Protein destruction by a helium atmospheric pressure glow discharge: capability and mechanisms.Journal of Applied Physics, 101 (7), article 074701, pp.1-9.Additional Information:• Biological sterilization represents one of the most exciting applications of atmospheric pressure glow discharges ͑APGD͒. Despite the fact that surgical instruments are contaminated by both microorganisms and proteinaceous matters, sterilization effects of APGD have so far been studied almost exclusively for microbial inactivation. This work presents the results of a detailed investigation of the capability of a helium-oxygen APGD to inactivate proteins deposited on stainless-steel surfaces. Using a laser-induced fluorescence technique for surface protein measurement, a maximum protein reduction of 4.5 logs is achieved by varying the amount of the oxygen admixture into the background helium gas. This corresponds to a minimum surface protein of 0.36 femtomole/ mm 2 . It is found that plasma reduction of surface-borne protein is through protein destruction and degradation, and that its typically biphasic reduction kinetics is influenced largely by the thickness profile of the surface protein. Also presented is a complementary study of possible APGD protein inactivation mechanisms. By interplaying the protein inactivation kinetics with optical emission spectroscopy, it is shown that the main protein-destructing agents are excited atomic oxygen ͑via the 777 and 844 nm emission channels͒ and excited nitride oxide ͑via the 226, 236, and 246 nm emission channels͒. It is also demonstrated that the most effective protein reduction is achieved possibly through a synergistic effect between atomic oxygen and nitride oxide. This study is a useful step toward a full confirmation of the efficacy of APGD as a sterilization technology for surgical instruments contaminated by prion proteins.

Fundamentally not requiring a vacuum chamber, atmospheric pressure glow discharges (APGDs) offer an exciting prospect for a wide range of material processing applications. To characterize their operation and establish their operation range, a radio frequency (rf) APGD is studied experimentally with measurement of discharge voltage, current, dissipated plasma power and plasma impedance. Different from the current understanding that rf APGD are operative only in the abnormal glow mode, we show the presence of two additional modes namely the normal glow mode and the recovery mode. It is shown that all three modes are spatially uniform and possess key characteristics of a glow discharge. So rf APGD have a much wider operation range than previously believed. To provide further insights, we investigate the transition from the abnormal glow mode to the recovery mode. It is established that the cause responsible for the mode transition is sheath breakdown, a phenomenon that is known in low- and moderate-pressure glow discharges but has not been reported before for atmospheric-pressure glow discharges. Finally we demonstrate that plasma dynamics, hence plasma stability, in these three modes are influenced crucially by the impedance matching between the plasma rig and the power source.

Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its ΔrecA, ΔrpoS, and ΔsoxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide.

It is well established that atmospheric pressure glow discharges are capable of bacterial inactivation. Much less known is their ability to destruct infectious proteins, even though surgical instruments are often contaminated by both bacteria and proteinaceous matters. In this letter, the authors present a study of protein destruction using a low-temperature atmospheric dielectric-barrier discharge jet. Clear evidences of protein removal are presented with data of several complimentary experiments using scanning electron microscopy, electron dispersive x-ray analysis, electrophoresis, laser-induced fluorescence microscopy, and protein reduction kinetics. Considerable degradation is observed of protein fragments that remain on their substrate surface after plasma treatment.

Current inactivation studies of Bacillus subtilis spores using atmospheric-pressure glow discharges (APGD) do not consider two important factors, namely microbial loading at the surface of a substrate and sporulation temperature. Yet these are known to affect significantly microbial resistance to heat and hydrogen peroxide. This letter investigates effects of microbial loading and sporulation temperature on spore resistance to APGD. It is shown that microbial loading can lead to a stacking structure as a protective shield against APGD treatment and that high sporulation temperature increases spore resistance by altering core water content and cross-linked muramic acid content of B. subtilis spores.

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