Physical Mechanisms of Inactivation of Bacillus subtilis Spores Using Cold Atmospheric Plasmas

Deng, X.T.; Shi, Jianjun; Kong, Michael G.

doi:10.1109/tps.2006.877739

Cited by 299 publications

(178 citation statements)

References 23 publications

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“…We also employed an UV passing glass ͑Thorlabs͒ to cover the protein sample so that only light of 170-400 nm could pass through. 21 The achieved reduction was about 7 picomole over 300 s, still much smaller than the 3 log reduction seen in Fig. 7͑a͒.…”

Section: -5mentioning

confidence: 65%

“…On the other hand, reactive oxygen species are known to play a dominant role for plasma microbial inactivation. 21 Therefore, it is desirable to add the oxygen gas into the working gas to provide an additional source for reactive oxygen species. For future reference, the oxygen gas added to the He flow through the powered electrode is referred to as the central O 2 flow and the auxiliary oxygen gas introduced downstream from the powered electrode is referred to as the side O 2 flow.…”

Section: Protein Reduction Kineticsmentioning

confidence: 99%

“…Factors responsible for protein reduction usually fall into five categories, namely ͑1͒ heat; ͑2͒ charged particles; ͑3͒ intense electric field; ͑4͒ UV photons; and ͑5͒ neutral reactive species, all commonly present in a gas discharge. These are known to contribute to microbial inactivation by APGD, 21 and could potentially be responsible for protein reduction observed in Figs. 4 and 5.…”

Section: -5mentioning

confidence: 99%

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Protein destruction by a helium atmospheric pressure glow discharge: Capability and mechanisms

Deng

Shi

Kong

2007

Journal of Applied Physics

171

105

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Citation: DENG X.T., SHI, J.J. and KONG, M.G., 2007. Protein destruction by a helium atmospheric pressure glow discharge: capability and mechanisms.Journal of Applied Physics, 101 (7), article 074701, pp.1-9.Additional Information:• Biological sterilization represents one of the most exciting applications of atmospheric pressure glow discharges ͑APGD͒. Despite the fact that surgical instruments are contaminated by both microorganisms and proteinaceous matters, sterilization effects of APGD have so far been studied almost exclusively for microbial inactivation. This work presents the results of a detailed investigation of the capability of a helium-oxygen APGD to inactivate proteins deposited on stainless-steel surfaces. Using a laser-induced fluorescence technique for surface protein measurement, a maximum protein reduction of 4.5 logs is achieved by varying the amount of the oxygen admixture into the background helium gas. This corresponds to a minimum surface protein of 0.36 femtomole/ mm 2 . It is found that plasma reduction of surface-borne protein is through protein destruction and degradation, and that its typically biphasic reduction kinetics is influenced largely by the thickness profile of the surface protein. Also presented is a complementary study of possible APGD protein inactivation mechanisms. By interplaying the protein inactivation kinetics with optical emission spectroscopy, it is shown that the main protein-destructing agents are excited atomic oxygen ͑via the 777 and 844 nm emission channels͒ and excited nitride oxide ͑via the 226, 236, and 246 nm emission channels͒. It is also demonstrated that the most effective protein reduction is achieved possibly through a synergistic effect between atomic oxygen and nitride oxide. This study is a useful step toward a full confirmation of the efficacy of APGD as a sterilization technology for surgical instruments contaminated by prion proteins.

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Section: -5mentioning

confidence: 65%

Section: Protein Reduction Kineticsmentioning

confidence: 99%

Section: -5mentioning

confidence: 99%

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Protein destruction by a helium atmospheric pressure glow discharge: Capability and mechanisms

Deng

Shi

Kong

2007

Journal of Applied Physics

171

105

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“…Studies using isolated protein and plasmid DNA models have shown protein and DNA damages [95]- [99], and more relevant experiments using bacterial cells have provided direct evidence of breaching and rapture of cell membrane [100], reduction and degradation of proteins [101]- [103], lipid damage [104], and (mostly) single-strand breaks (SSB) of DNA [105]. These results have prompted the suggestion of the involvement of ROS, charged particles, and UV photons [92][100] [105] - [108], with ROS often linked to oxidation of protein and lipid and with UV photons linked to DNA damages.…”

Section: Reactive Plasma Species and Their Biological Targetsmentioning

confidence: 99%

Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Kong¹,

Keidar²,

Ostrikov³

2011

J. Phys. D: Appl. Phys.

107

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To cite this version:M G Kong, M Keidar, K Ostrikov. Plasmas meet nanoparticleswhere synergies can advance the frontier of medicine. Journal of Physics D: Applied Physics, IOP Publishing, 2011, 44 (17) AbstractNanoparticles and low-temperature plasmas have been developed, independently and often along different routes, to tackle the same set of challenges in biomedicine. There are intriguing similarities and contrasts in their interactions with cells and living tissues, and these are reflected directly in the characteristics and scope of their intended therapeutic solutions, in particular their chemical reactivity, selectivity against pathogens and cancer cells, safety to healthy cells and tissues, and targeted delivery to diseased tissues. Time has come to ask the inevitable question of possible plasma-nanoparticle synergy and the related benefits to the development of effective, selective, and safe therapies for modern medicine. This perspective article offers a detailed review of the strengths and weakenesses of nanomedicine and plasma medicine as a stand-alone technology, and then provides a critical analysis of some of the major opportunities enabled by synergising the nanotechnology and the plasma technology. It is shown that the plasma-nanoparticle synergy is best captured through the plasma nanotechnology and its benefits for medicine are highly promising.

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“…[7][8][9][10][11][12] Much has been learnt of the bactericidal capability and related physical mechanisms of low-temperature plasmas. 13 By contrast, there are very few reports on their ability to destruct proteinaceous matters [14][15][16] and all reported are limited to the use of vacuum plasmas. More significantly, medical sterilization involves both bacterial inactivation and protein destruction.…”

mentioning

confidence: 99%

Protein destruction by atmospheric pressure glow discharges

Deng

Shi

Chen

et al. 2007

Applied Physics Letters

161

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It is well established that atmospheric pressure glow discharges are capable of bacterial inactivation. Much less known is their ability to destruct infectious proteins, even though surgical instruments are often contaminated by both bacteria and proteinaceous matters. In this letter, the authors present a study of protein destruction using a low-temperature atmospheric dielectric-barrier discharge jet. Clear evidences of protein removal are presented with data of several complimentary experiments using scanning electron microscopy, electron dispersive x-ray analysis, electrophoresis, laser-induced fluorescence microscopy, and protein reduction kinetics. Considerable degradation is observed of protein fragments that remain on their substrate surface after plasma treatment.

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Physical Mechanisms of Inactivation of Bacillus subtilis Spores Using Cold Atmospheric Plasmas

Cited by 299 publications

References 23 publications

Protein destruction by a helium atmospheric pressure glow discharge: Capability and mechanisms

Protein destruction by a helium atmospheric pressure glow discharge: Capability and mechanisms

Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Protein destruction by atmospheric pressure glow discharges

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