No abstract

To explore the effect of concentrated dephenolic cottonseed protein (CDCP) partly instead of fish meal on the non-specific immune of Penaeus monodon, we designed a single factor test to study the four types of non-specific immunity of P. monodon hepatopancreas after acute salinity stress. A total number of 5,400 P. monodon were selected with an initial body weight of 1.76±0.15g and randomly divide into 6 groups (3 replicates per group, 900 tails per replicate). Shrimp were feed with two protein level diets (Diet A and Diet B). After 8 weeks of feeding, P. monodon were randomly selected with an average weight of 14.71±1.06g in each feed group, and subjected to acute salinity stress of 10ppt and 20ppt after temporary rearing, and the heat shock protein 70 (HSP70), Na+-K+-ATPase, cortisol and the content of alkaline phosphatase (ALP) were measured. The results of the study showed that when the CDCP partially replaced fish meal of P. monodon diet, the hepatopancreas HSP70, and the contents of Na+-K+-ATPase and cortisol were significantly higher than those of the control group. In the acute salinity change, there was no significant difference in the survival rate of P. monodon at 48h under the two salinity stresses, and the four immune indexes showed different trends. The expression level of Cortisol was more prominent than that of HSP70, which could better reflect the stress degree of P. monodon. Most of the indicators showed a trend of rising and then falling and then rising compared with the original level, and the changes of the four indicators of hepatopancreas in the Diet B group were significantly higher than those in the Diet A group at most time point of the two salinities.

The objective of the present work was to evaluate the effects of different levels of vitamin E in feed on the spermatophore regeneration and quality of male Penaeus monodon. The experiment was carried out with the four following treatments: the basal diet no added vitamin E, the diet added 200, 600 and 1,000 mg/kg respec-How to cite this article: Jiang S, Liu D, Zhou F, et al. Effect of vitamin E on spermatophore regeneration and quality of pond-reared, black tiger shrimp (Penaeus monodon). Aquac

An experimental study of polyculture of sea cucumber Holothuria scabra with Pacific white shrimp Litopenaeus vannamei was carried out in the laboratory. Results indicated that nutrient structure of the culture system was influenced significantly by the sea cucumber: the phosphate and nitrate concentrations were enhanced more obviously in the polyculture system (PS) than in the monoculture system (MS), but by contrast, the ammonium concentration remained low in the PS. The sulfide contents of the sediment were reduced in the PS to 10.32±1.85mg/kg, accounting for about 2/5 of that in the MS. The PS of sea cucumbers had positive effects on the growth and survival of shrimp, the specific growth rate of weight of shrimp in the PS was 2.89±0.63%/day and significantly higher than that of the MS (average 2.05±0.57%/d). Survival rate of the shrimp in PS was 79.3±22.9%, dramatically higher than that of the MS (average 64.0±17.5%). In the PS, the shrimp had no negative effect on the sea cucumbers who ingested and assimilated food residue in the sediment of the culture system with an assimilation efficiency of 42.06±10.62% for organic matter. PS of sea cucumber Holothuria scabra in the shrimp culture system had both environmental and economic benefits. This PS method could contribute to sustainable development of aquaculture in China.The IJA appears exclusively as a peer-reviewed on-line open-access journal at http://www.siamb.org.il/. To read papers free of charge, please register online at registration form. Sale of IJA papers is strictly forbidden.

Authors' Contribution MQ conceived the idea, performed the experiments, wrote manuscript and compiled the first draft. MW and FC were the members of supervisory committee. MT analyzed the data. SF was lab incharge. AM supervised in Max-Planck Institute.ZA assisted and guided in lab work performed. MH helped in manuscript writing.ARA designed and susupervised the study.