A dedicated analytical scanning transmission electron microscope (STEM) with dual energy dispersive spectroscopy (EDS) detectors has been designed for complementary high performance imaging as well as high sensitivity elemental analysis and mapping of biological structures. The performance of this new design, based on a Hitachi HD-2300A model, was evaluated using a variety of biological specimens. With three imaging detectors, both the surface and internal structure of cells can be examined simultaneously. The whole-cell elemental mapping, especially of heavier metal species that have low cross-section for electron energy loss spectroscopy (EELS), can be faithfully obtained. Optimization of STEM imaging conditions is applied to thick sections as well as thin sections of biological cells under low-dose conditions at room- and cryogenic temperatures. Such multimodal capabilities applied to soft/biological structures usher a new era for analytical studies in biological systems.
A new kind of microstrip line on which the spoof surface plasmon polaritons (SPPs) can propagate at low frequencies (such as microwave or terahertz regime) is developed. The upper metal-strip is designed by introducing subwavelength periodically inward openings on the edges. Numerical methods are used to analyse the dispersion relation and the asymptotic frequency. It is verified that such periodically structured microstrip lines can support spoof SPPs in the frequency range between 200 MHz and 8 GHz. Compared with the quasi-TEM modes on conventional metal-strip lines, the spoof SPPs can be highly localised on the surface of the structured microstrip lines, so that the crosstalk between the different structured microstrip lines is very weak. Therefore this new kind of periodically structured microstrip line would be of great use in high density microwave circuits or high-speed systems.
Flexoelectricity possesses two gradient-dependent electromechanical coupling effects: the direct flexoelectric effect and the converse flexoelectric effect. The former can be used for sensing and energy generation; the latter can be used for ultraprecision actuation and control applications. Due to the direct flexoelectricity and large deformations, theoretical fundamentals of a generic nonlinear distributed flexoelectric double-curvature shell energy harvester are proposed and evaluated in this study. The generic flexoelectric shell energy harvester is made of an elastic double-curvature shell laminated with flexoelectric patches and the shell experiences large oscillations, such that the von Karman geometric nonlinearity occurs. Flexoelectric output voltages and energies across a resistive load are evaluated using the current model in the closed-circuit condition when the shell is subjected to harmonic excitations and its steady-state voltage and power outputs are also calculated. The generic flexoelectric shell energy harvesting theory can be simplified to shell (e.g., cylindrical, conical, spherical, paraboloidal, etc.) and nonshell (beam, plate, ring, arch, etc.) distributed harvesters and the simplification procedures are demonstrated in three cases, i.e., a cylindrical shell, a circular ring and a beam harvester. Other shell and nonshell flexoelectric energy harvesters with standard geometries can also be defined using their distinct two Lamé parameters and two curvature radii.
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