A method is presented whereby the toxic amino acid gizzerosine can be determined in j s h meals to a lower limit of 10 mg k g -' . Fish meal was hydrolysed with hydrochloric acid and, after concentration by fieeze drying, derivatised with dansyl chloride solution without further clean-up. A ,@action containing the tridansyl derivative of gizzerosine was separated and collected by HPLC and j?om this the didansyl derivative was separated a f e r treatment with formic acid on the same column as previously using the same mobile phase. Seventeen fish meals, some causing severe gizzard erosion in chickens, were analysed but in none of them could gizzerosine at or nbove this level be found.
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