Summary. The uptake of phenol by pure cultures of Psc~rrdomonas prrridtr growing on phenol in continuous culture has been studied. The purpose of the experiments was to determine the kinetic parameters governing uptake of phenol by organisms growing on phenol in the highconversion range by measuring uptake rates per unit biomass per unit time at various phenol concentrations. The microorganisms used were taken from a chemostat at residence times of 8. 5.25, 3.85, 3.2, 3. and 2.7 h. The Monod-Haldane model and modifications of it were applied to the data and the best kinetic parameters were determined by nonlinear leastsquares techniques. The bsst model was a two-parameter simplification of Monod-Haldane in which I* = K I S / ( K z + S'), The value of K , was found to increase monotonically with the value of phenol concentration in the original chemostat with an apparent induction "threshold" of 0. I mg/L.

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The specific uptake rate of phenol by washed cells of Pseudomonas putida grown on phenol in steady-state continuous culture at various dilution rates was studied. The Monod-Haldane-type equation was applied to fit the data and the best kinetic parameters were determined by nonlinear least-squares techniques. The values of the kinetic parameters were found to increase monotonically with the phenol concentration in the original chemostat. The relations between the values of kinetic parameters and phenol concentration in the chemostat were described by empirical equations. Then the equation governing the instant uptake of phenol by microorganisms in chemostat in the high conversion range of phenol was proposed. This equation together with the mass balance equations can be used to determine the stability range of continuous stirred tank biochemical reactors (CSTBR) utilizing phenol.

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