Abstract. Samiyarsih S, Fitrianto N, Azizah E, Herawati W, Rochmatino. 2020. Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers. Biodiversitas 21: 1755-1766. Indonesia has developed a food consumption diversification based on local sources. Sweet potato (Ipomoea batatas) is one of the tubers of carbohydrate sources in addition to wheat, rice, corn, potatoes, and cassava. It has a wide distribution, and high cultivars diversity produces a high variation on its morphology, anatomy, and genetic modification. The objectives of this research were to investigate the anatomical profile and genetic variability of eight sweet potatoes (Ipomoea batatas) cultivars found in Banyumas, Central Java, Indonesia. Anatomical profile was observed by the embedding method and subjected to analysis of variance. DNA amplification using Random Amplified Polymorphic DNA (RAPD) method with 10 primers, namely OPA-1, OPA-2, OPA-3, OPA-4, OPA-9, OPA-11, OPA-13, OPA-15, OPA-16, and OPA-18. The result of the anatomical study showed that eight sweet potato cultivars have significantly different (p<0.05) variation in leaf anatomical profile. RAPD primers produced 52 amplified fragments varying from 100 to 1300 bp in size, and 90.40% of the amplification bands were polymorphic. The genetic similarity level is ranged from 0.37 to 0.93. Two unspecific groups were forming at a coefficient of 55% from the dendrogram. The first group consisted of one cultivar, Cangkuang, and the second group consisted of seven cultivars Antin, Ungu Tua, Borobudur, Sukuh, Sari, Beta, and Kidal. The nearest relationships cultivars were Borobudur, and Ungu Tua had the highest similarity coefficient of 93%, and the lowest similarity found in Cangkuang and Antin with a coefficient of 37%. The range of genetic distance of eight cultivars was from 0.37-0.93. Implementation of the research would particularly useful for the identification and evaluation of the genetic improvement of sweet potato cultivars.
Herawati W, Amurwanto A, Nafi’ah Z, Ningrum AM, Samiyarsih S. 2018. Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity. Biodiversitas 19: 119-125. A variation analysis of three salak cultivars was conducted in Banyumas District, Central Java, Indonesia. The aim of the study was to investigate variation amongst the cultivars based on anatomy and genetic diversity by using RAPD (Random Amplified Polymorphic DNA) technique. The result of the study showed that all salak cultivars have similar variation in leaf anatomy. The only distinguishing characteristic is the number of trichomes on cultivars Kedung Paruk and Kalisube per 1 mm2 unit of leaf area, while the cultivars of Candinegara lacked trichomes. Ten primers could detect 50 polymorphic bands (80,6%) from a total of 62 bands which could be observed clearly. The percentage of polymorphic loci in 'Candinegara' was 64%, 'Kedung Paruk' 62%, and 'Kalisube' 44%. The heterozygosity value of 'Candinegara' was 0.1590, 'Kedung Paruk' 0,1449, and 'Candinegara' (0.0235). Meanwhile, the furthest genetic distance was shown by 'Kedung Paruk' and ‘Candinegara’ (0.0704). This result gives us important basic information about cultivars of Salacca zalacca in Banyumas District which have high genetic diversity and germplasm. These results can be used for further research such as for conservation and genetic engineering.
Kewirausahaan merupakan bidang yang masih kurang diminati oleh kalangan remaja.walaupun upaya yang sudah dilaksanakan oleh pemerintah dan pihak swasta sudah banyak, namun masih perlu dukungan banyak pihak, Tujuan kegiatan pengabdian kepada masyarakat ini untuk menanamkan jiwa kewirausahaan sejak dini pada siswa. Diharapkan pada waktu yang akan datang para siswa menjadi wirausahawan yang handal dan professional.Kegiatan ini dilakukan oleh team pengabdi masyarakat Fakultas Ekonomi dan Bisnis Universitas Wijaya Kusuma Surabaya dan bekerja sama dengan Kepala Sekolah SMKKartini Surabaya, dari bulan Maret 2021 sampai bulan Agustus 2021. Jumlah siswa yang dibina dalam kewirausahaan sebanyak 35 orang, metode pelaksanaan kegiatan ini menggunakan pendekatan pelatihan kewirausahaan disertai contoh pembuatan produk makanan frozen. Adapun pelaksanaan kegiatan pelatihan dilakukan dengan pemberian materimembangun jiwa kewirausahaan.
Background - Competition in the Batik industry in Indonesia is quite sharp. Therefore it is necessary to apply the right marketing strategy in this industry. Whereas each batik industry that originates from the region has its own uniqueness that can attract the attention of consumers. Therefore, a positioning strategy for batik products is needed to be able to create more value in the minds of consumers compared to its competitors. Purpose - This research purposed to describe the position of competition (positioning) on batik industry in East Java based on the perceptions of local batik consumers. Design / Methodology / Approach - The study took samples of batik consumers in 9 cities in East Java, namely Bangkalan, Sidoarjo, Lamongan, Tuban, Pasuruan, Tulungagung, Mojokerto, Ponorogo and Banyuwangi. The population was taken by using purposive sampling technique. Respondents were taken as many as 200 respondents from selected batik producing cities. The research was conducted on 9 kinds of attributes that distinguish the distinctive characteristics of batik between regions in East Java including the quality of the fabric used, the quality of the coloring of the fabrics used, the patterns or motifs, the various colors, prices, design innovation, popularity, value art and intrinsic meaning. To conduct the analysis, analysis techniques using multidimensional scaling were used. Results and Discussion - The results showed a significant difference between the batik in each region which shows the characteristics of the area. The implication of this research was very useful for batik business in East Java to find out their competitive position among this industry in East Java, so that they can determine the right competition strategy according to the advantages or characteristics of each region. Conclusion - There are differences in perceptions of the quality attributes of the fabric material, the quality attributes of fabric coloring, batik patterns or motifs, various colors, prices, design innovation, popularity, artistic value and intrinsic meaning of batik batik in East Java which is the object of this research. Research implication - The research contributions are presented in the form of theoretical contributions and practical contributions. The theoretical contribution made is that this study supports previous research, that marketers can find out the competitive position in the industry through the perceived attributes of consumers. The implication of this research is very useful for marketers to find out who their close competitors are so that marketers can apply the right strategy to deal with competitors. Batik entrepreneurs or craftsmen should make products with high artistic value that characterize the Indonesian culture. Research limitations – The limitation of this study lies in the sample selection, because consumers only know the product attributes that they know, in the next research, consumer selection should preferably be on consumers who know all the characteristics of the businesses being compared. Further research can also examine the segmentation, targeting and positioning policies of batik products in marketing their products in the community.
Lansium parasiticum (Duku) is a plant species with complex morphological characters because of its wide distribution. This study examined the plant anatomical variation patterns and phenetic relationships among 15 local duku samples from Purwokerto, Purbalingga, and Banjarnegara. The anatomical data were analyzed descriptively, and the similarity relationship was measured with the UPGMA method using the MEGA 10.0.1 software. The results of anatomical characters analysis suggested a high degree of diversity among the duku. UPGMA tree derived from cluster analysis showed two majors clusters. The first cluster consisted of samples whose upper epidermis thickness was ⩾16.5µm. The second cluster comprised fourteen samples with upper epidermis thickness <16µm.
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