Malassezia yeasts have recently gained medical importance as emerging pathogens associated with a wide range of dermatological and systemic infections. Since standardized methods for in vitro antifungal susceptibility testing have not yet been established for Malassezia spp., related diseases are always treated empirically. As a result, a high rate of recurrence and decreased antifungal susceptibility have appeared. Thus, the aims of the study were to assess and analyze the in vitro susceptibility of Malassezia isolated from pityriasis versicolor (PV) lesions and healthy controls. A total of 58 Malassezia strains isolated from PV patients and healthy controls were tested. In vitro antifungal susceptibility testing was conducted using the CLSI broth microdilution with some modifications. Candida spp. criteria established in accordance with CLSI guidelines were used for data interpretation. Ketoconazole and posaconazole seemed to be the most effective molecules against Malassezia species. However, considerable percentages of itraconazole, fluconazole, and amphotericin B ‘‘resistant’’ strains (27.6%, 29.3%, and 43.1%, respectively) were revealed in this study. Malassezia furfur, M. sympodialis, and M. globosa showed different susceptibility profiles to the drugs tested. These results emphasize the importance of accurately identifying and evaluating the antifungal susceptibility of Malassezia species in order to guide a specific and effective treatment regimen.
Over the last decade, Malassezia species have emerged as increasingly important pathogens associated with a wide range of dermatological disorders and bloodstream infections. The pathogenesis of Malassezia yeasts is not completely clear but it seems to be strictly related to Malassezia strains and hosts and need to be better investigated. This study aimed to assess the enzymatic activities, biofilm formation and in vitro antifungal profiles of Malassezia spp. from Pityriasis versicolor and heathy patients. The potential relationship between virulence attributes, the antifungal profiles and the origin of strains were also assessed. A total of 44 Malassezia strains isolated from patients with (n = 31) and without (n = 13) Pityriasis versicolor (PV) were employed to evaluate phospholipase (Pz), lipase (Lz), hemolytic (Hz) activities and biofilm formation. In addition, in vitro antifungal susceptibility testing was conducted using the CLSI broth microdilution with some modifications. A high percentage of strains produced phospholipase, lipase, hemolysins and biofilm regardless of their clinical origin. The highest number of strains producing high enzymatic activities came from PV patients. A correlation between the intensity of hydrolytic activities (lipase and phospholipase activities) and the hemolytic activity was detected. Positive associations between Lz and the low fluconazole susceptibility and Hz and biofilm formation were observed. These results suggest that enzyme patterns and biofilm formation together with antifungal profiles play a role in the pathogenicity of Malassezia spp. and might explain the implication of some Malassezia spp. in invasive fungal infections and in the development of inflammation.
In spite of the increasing medical interest of Malassezia yeasts, the virulence factors of M. furfur causing blood stream infections (BSI) were never investigated. Therefore, phospholipase (Pz), lipase (Lz), hemolysin (Hz), biofilm production and in vitro antifungal susceptibility profiles were evaluated in M. furfur strains, both isolated from pityriasis versicolor (PV) patients (n = 18; Group 1) or from preterm infants BSI (n = 21; Group 2). All the teststains exhibited Pz activity whereas 92.3% and 97.4% of strains exhibited Lz and Hz activities, respectively. Pz, Lz and Hz activities were higher (i.e., lower values) within Group 1 strains (i.e., 0.48, 0.40 and 0.77) than those within Group 2 (i.e., 0.54, 0.54 and 0.81). The biofilm production was higher within Malassezia isolates from Group 2 (0.95 ±0.3) than from Group 1 (0.72±0.4). Itraconazole and posaconazole were the most active drugs against M. furfur, followed by amphotericin B and fluconazole. The minimum inhibitory concentrations (MIC) values varied according to the origin of M. furfur strains being statistically lower in M. furfur from Group 1 than from Group 2. This study suggests that M. furfur strains produce hydrolytic enzymes and biofilm when causing PV and BSI. Data suggest that the phospholipase activity, biofilm production and a reduced antifungal susceptibility profile might favour M. furfur BSI, whereas lipase and haemolytic activities might display synergic role in skin infection.
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