Abstrak-Kabupaten Banyumas memiliki banyak sungai yang potensial untuk dikembangkan Pembangkit Listrik Tenaga Mikrohidro (PLTMH). Konsep listrik berbasis mikrohidro dengan memanfaatkan sumber air yang berada di sekitar pemukiman. Tujuan penelitian ini membuat peta potensi sungai di wilayah Kabupaten Banyumas untuk dikembangkan PLTMH skala 100-500 kW, membuat rancangan dan prototipe PLTMH skala laboratorium serta uji performansi. Pelaksanaan penelitian meliputi: pengumpulan data iklim, pengukuran debit sungai dan ketinggian terjunan; Pembuatan prototipe turbin cross-flow; serta uji performansi protipe di Laboratorium Teknik Sistem Termal dan Energi Terbarukan Fakultas Pertanian Universitas Jenderal Soedirman. Hasil penelitian menunjukan bahwa rancangan turbin cross-flow belum menunjukkan performansi optimum, tetapi peralatan transmisi dapat bekerja baik, sistem penyaluran energi bekerja cukup baik memutar turbin pada kondisi dengan maupun tanpa beban. Hasil pengujian menggunakan kekuatan motor pompa penuh didapatkan hasil 1.114,7 rpm generator; voltase 77,7 V; dan arus 0,5 A pada saat tanpa beban, serta 1.018,6 rpm generator; voltase 70,2 V, dan arus 0,5 A pada saat diberi beban lampu LED 5 W.Kata kunci -Mikrohidro, PLTMH, turbin, cross-flow, transmisi.Abstract -Banyumas District has a lot of potential rivers, to develop Micro Hydro Power Plant (MHP). The concept of MHP is to utilize water sources located around the settlement. The purpose of the study are: to create a map of potential river in the district of Banyumas to develop MHP scale 100-500 kW; create a design and prototype laboratory scale; and conduct performance test laboratory. Implementation of the research include: climate data collection, measurement of flow rate and head; prototyping cross-flow turbine types, and performance test of the prototype MHP conducted at the Laboratory of Thermal Systems Engineering and Renewable Energy, Faculty of Agriculture, Unsoed. The research results of a cross-flow turbine has been designed not show a good performance, but the transmission equipment can work, and the energy distribution could turn turbines, both on the condition of using and without load. Test results using the full power of the pump motor when no load is obtained 1114.7 rpm generator; voltage of 77.7 V; and a current of 0.5 A, whereas when using the 5 W LED lamp load generated 1018.6 rpm generator; voltage of 70.2 V and a current of 0.5 A.