High variability in the assessed pharmacokinetic parameters was observed, with a 38% variation in C(ss) between TD and conditioning regimen; Cl(T)/F decreased by 30%, suggesting drug accumulation after multiple-dose regimen. Although being lower than reported in the literature, this variation may be associated with toxicity of the proposed treatment, justifying patient monitoring and enhancing validity of previous pharmacokinetic evaluation using TD regimen.
In this work, we describe the identification of desvenlafaxine in extended release capsules of venlafaxine (VEN) in acid degradation studies. We developed a stability indicating reverse-phase HPLC method and validated for the analysis of VEN in pharmaceutical formulation. The HPLC method was linear over the range of 0.45-1.05 mg/ml (r 2 =0.999). The RSD values for intra-and inter-day precision studies showed good results (RSD < 2%) and accuracy was greater than 99%. The degradation studies in acidic media for 24 h showed two additional peaks, which were further identified by ESI-MS/MS as the desvenlafaxine and the dehydration product of venlafaxine. Furthermore, desvenlafaxine is the major active metabolite of venlafaxine and has recently been approved for treatment of major depressive disorder.
Cinnarizine (CIN) is used in the treatment of cerebral circulatory, peripheral circulatory and balance disorders. An analytical method was developed and validated to determine the dissolution of cinnarizine (CIN) in capsules compounded by ultraviolet detection spectrophotometry. The resolutions of the Agência Nacional de Vigilância Sanitária nº. 899 of May 29, 2003 and nº. 166 of July 24, 2017 were used as the guide for validation of analytical methods. CIN showed high solubility in 0.1 mol.L-1 hydrochloric acid at 37 ° C (9.43 mg.mL-1), selectivity / specificity in the presence of other components (0.35 %), maximum absorption spectrum in 251 nm; linearity (r = 0.9999); precision (RSD repet.: 0.09%, RSD inter.: 1.10%); accuracy (LQC = 99.9%, MQC = 99.5%, HQC = 100.7%); limits of detection and quantification of 0.042 and 0.42 μg.mL-1, respectively. In the parameter robustness it has been proven that the proposed method does not suffer significant variations. The validated analytical method is easy to perform and has reliable selectivity / specificity, linearity, precision, accuracy, limits of detection, limits of quantification and robustness. Therefore, the ultraviolet detection spectrophotometry method is applicable to determine CIN in capsules compounded in simple, accurate and low cost dissolution assays.
Background Imatinib mesylate (IM) treatment adherence is a challenge, especially in an economic-social population neglected from developing countries. Objective To create a new complementary audiovisual educational intervention model to improve IM treatment adherence of CML patients. Methods Two adherence verification methods were applied before and after intervention: modified Morisky-Green test and molecular responses (BCR-ABL transcripts quantification). Adherence estimates were calculated using univariate and multivariate component analysis (MCA) for the socio-demographic and clinical characteristics of patients. Results Modified Morisky-Green test results demonstrated a substantial increase of CML patient adherence from 23% (pre-film) to 65% (post-film). Greater improvement was obtained for patients presenting major molecular response (MMR) from 38% (pre-film) to 60% (post-film). Although slight gain for complete molecular response (CMR) from 23% (pre-film) to 26% (post-film) was achieved, it represents a total tumour regression. MCA identified that females <50 years-old, using less than two medications (no disease associated) and CMR condition were the most benefited with intervention. Conclusion Audiovisual educational intervention was an effective complementary pro-adherence model, activating patient memory and improving IM treatment adherence. Although this intervention shows effective, not all patients responded as expected, being necessary a combination of educational and clinical interventions to improve IM adherence.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.