Background Melanin is a natural pigment that can be applied in different fields such as medicine, environment, pharmaceutical, and nanotechnology. Studies carried out previously showed that the melanin produced by the mel1 mutant from Aspergillus nidulans exhibits antioxidant, anti-inflammatory, and antimicrobial activities, without any cytotoxic or mutagenic effect. These results taken together suggest the potential application of melanin from A. nidulans in the pharmaceutical industry. In this context, this study aimed to evaluate the effect of factors L-tyrosine, glucose, glutamic acid, l-DOPA, and copper on melanin production by the mel1 mutant and to establish the optimal concentration of these factors to maximize melanin production. Results The results showed that l-DOPA, glucose, and copper sulfate significantly affected melanin production, where l-DOPA was the only factor that exerted a positive effect on melanin yield. Besides, the tyrosinase activity was higher in the presence of l-DOPA, considered a substrate required for enzyme activation, this would explain the increased production of melanin in this condition. After establishing the optimal concentrations of the analyzed factors, the melanin synthesis was increased by 640% compared to the previous studies. Conclusions This study contributed to elucidating the mechanisms involved in melanin synthesis in A. nidulans as well as to determining the optimal composition of the culture medium for greater melanin production that will make it possible to scale the process for a future biotechnological application.
Background Melanin is a natural pigment that can be applied in different fields such as medicine, environment, pharmaceutical, and nanotechnology. Studies carried out previously showed that the melanin produced by the MEL1 mutant from Aspergillus nidulans exhibits antioxidant, anti-inflammatory, and antimicrobial activities, without any cytotoxic or mutagenic effect. These results taken together suggest the potential application of melanin from A. nidulans in the pharmaceutical industry. In this context, this study aimed to evaluate the effect of factors L-tyrosine, glucose, glutamic acid, L-DOPA, and copper on melanin production by the MEL1 mutant and to establish the optimal concentration of these factors to maximize melanin production. Results The results showed that L-DOPA, glucose, and copper sulfate significantly affected melanin production, where L-DOPA was the only factor that exerted a positive effect on melanin yield. Besides, the tyrosinase activity was higher in the presence of L-DOPA, considered a substrate required for enzyme activation, this would explain the increased production of melanin in this condition. After establishing the optimal concentrations of the analyzed factors, the melanin synthesis was increased by 640% compared to the previous studies. Conclusions This study contributed to elucidating the mechanisms involved in melanin synthesis in A. nidulans as well as to determining the optimal composition of the culture medium for greater melanin production that will make it possible to scale the process for a future biotechnological application.
Cigarette product waste contains toxic chemicals, including human carcinogens, which leach into and accumulate in the environment and represent a current environmental problem neglected for too long. This study aimed to select filamentous fungi capable of decreasing tobacco extract toxicity as an alternative to a future bioremediation process. The 38 isolates obtained from Culture collection of microorganisms to biotechnological and environmental importance-CCMIBA (Brazil) were cultivated in yeast extract (10 g.L À1) and dextrose (10 g.L À1) containing cigarette tobacco extract (200 mL.L À1) for seven days at 28 C on a rotary shaker at 150 rpm. The fungal growth rate was determined to infer fungal tolerance to tobacco extract, and supernatants from cultivated fungi were used to run the toxicity test using Allium cepa assay. The Fusarium sp. strain I.17, isolated from cigarette waste, was the only lineage capable of growing in 20% (v/v) of cigarette tobacco extract, allowed the onions to root, and was selected for optimization. Initially, for the experimental design to selected fungus, a fractional factorial experimental design 2 5À1 was used to examine the effects of yeast extract, cigarette tobacco extract concentration, dextrose, copper sulfate and pH fungal cultivation. The supernatants of these assays were used to run the toxicity test, and yeast extract and copper sulfate were statistically significant in the fungal growth for the decreasing toxicity process and this variable as were select to central composite design. The highest concentration of yeast extract negatively influenced the toxicity decrease, 0.5% of yeast extract in the culture media is the maximum concentration to achieve the best result and to copper sulfate the best result was using 10 μmol.L À1. In conclusion, the experimental design optimized more than seven times the efficiency of tobacco toxicity reducing, resulting in more than 50% of onion root growth, demonstrating the methodology success. And ITS region was used to taxonomy and molecular phylogeny of the isolate Fusarium sp. strain I.17. These results suggest that Fusarium sp. strain I.17 can be used as a potential microorganism to toxicity treatment of cigarette wastes, minimizing the environmental impact of direct burning.
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