Cigarette product waste contains toxic chemicals, including human carcinogens, which leach into and accumulate in the environment and represent a current environmental problem neglected for too long. This study aimed to select filamentous fungi capable of decreasing tobacco extract toxicity as an alternative to a future bioremediation process. The 38 isolates obtained from Culture collection of microorganisms to biotechnological and environmental importance-CCMIBA (Brazil) were cultivated in yeast extract (10 g.L À1) and dextrose (10 g.L À1) containing cigarette tobacco extract (200 mL.L À1) for seven days at 28 C on a rotary shaker at 150 rpm. The fungal growth rate was determined to infer fungal tolerance to tobacco extract, and supernatants from cultivated fungi were used to run the toxicity test using Allium cepa assay. The Fusarium sp. strain I.17, isolated from cigarette waste, was the only lineage capable of growing in 20% (v/v) of cigarette tobacco extract, allowed the onions to root, and was selected for optimization. Initially, for the experimental design to selected fungus, a fractional factorial experimental design 2 5À1 was used to examine the effects of yeast extract, cigarette tobacco extract concentration, dextrose, copper sulfate and pH fungal cultivation. The supernatants of these assays were used to run the toxicity test, and yeast extract and copper sulfate were statistically significant in the fungal growth for the decreasing toxicity process and this variable as were select to central composite design. The highest concentration of yeast extract negatively influenced the toxicity decrease, 0.5% of yeast extract in the culture media is the maximum concentration to achieve the best result and to copper sulfate the best result was using 10 μmol.L À1. In conclusion, the experimental design optimized more than seven times the efficiency of tobacco toxicity reducing, resulting in more than 50% of onion root growth, demonstrating the methodology success. And ITS region was used to taxonomy and molecular phylogeny of the isolate Fusarium sp. strain I.17. These results suggest that Fusarium sp. strain I.17 can be used as a potential microorganism to toxicity treatment of cigarette wastes, minimizing the environmental impact of direct burning.
Background Changes in the fungal community in the litter decomposition by invasive plants can negatively impact nutrient cycling in natural ecosystems. One still does not know the dimension of this hypothesis, but apparently, it is not despicable. This study evaluated the assemblage composition of fungi during litter decomposition in areas of Atlantic Forest invaded or not invaded by Tradescantia zebrina using Illumina MiSeq and metabarcoding analysis. Results The invaded sample showed significantly higher richness and a difference in the species dominance than the invaded litter. Ascomycota was the first most abundant phylum in both areas. Even so, the dissimilarity between areas can be evidenced. The fungal from Basidiomycota were very representative in the non-invaded areas (ranged from an abundance of 43.29% in the non-invaded to 2.35% in the invaded sample). The genus Lepiota can indicate the primary functional group related to biomass degradation and showed the might difference about the invaded areas due to its essential reduction by the invader. In the invaded sample, there was a total absence of the endophyte-undefined saprotroph guild. Also, some genera not taxonomically characterized were eliminated in the invaded sample, revealing that the fungal biodiversity of areas has not yet been thoroughly characterized. Conclusions Hence, makes impossible the real interpretation of the invasive plant impact, showing the importance of continuing research on fungal biodiversity. It is important to emphasize that the replacement of the native species by T. zebrina may be responsible for the elimination of fungal groups that have not yet been identified.
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