Although a calcium hydroxide paste was placed in the prepared canals, the number of positive canals had increased in the period between visits. However, the number of microorganisms had only increased to 0.93% of the original number of CFU (sample 1). It is concluded that a calcium hydroxide and sterile saline slurry limits but does not totally prevent regrowth of endodontic bacteria.
Two sets of teeth with apical periodontitis were collected at different geographic locations to study the identity of bacteria left in the root dentinal tubules. Root dentin of 20 of these teeth was cultured from three locations between pulp and cementum (A, B, and C). In addition dentin from eight teeth was examined histologically. Using the culturing technique bacteria were found in 77% of the dentin samples from set 1 (Amsterdam) and in 87.5% of the dentin samples from set 2 (Glasgow). At greater distance, in layer C, from the pulp bacteria were found in 62% (13 of 21) of the dentin samples. Twenty-three percent (3 of 13) of set 1 and 25% (2 of 8) of set 2 contained >50,000 colony-forming units/mg of dentin in layer C. In layers closer to the pulp higher numbers of anaerobic bacteria and gram-positive rods were found, as well as a larger number of bacterial species. Histological sections showed bacterial penetration in dentinal tubules in 5 of 8 teeth. In the other three teeth where the colony-forming units/mg recovered was <10,000, no histological signs of tubule penetration was seen. It seems clear that, in more than half of the infected roots, bacteria are present in the deep dentin close to the cementum and that anaerobic culturing of dentin is more sensitive than histology to detect these bacteria.
Objectives: The aim of this study was to assess the influence of scan setting selection, including field of view (FOV) ranging from small to large, number of projections and scan modes on the visibility of the root canal with cone beam CT (CBCT). Methods: One human mandible cadaver was scanned with CBCT (Accuitomo 170; J Morita MPG Corp., Kyoto, Japan) using six different FOVs (464 cm, 666 cm, 868 cm, 10610 cm, 14610 cm and 17612 cm) with either 360 or 180 projections in standard and high resolution. The right canine was selected for evaluation. Ten observers independently assessed the visibility of the canal space and overall image quality on a five-point scale.Results: The results indicate that both selections of FOV and number of projections have significant influence on root canal visibility (p 5 0.0001), whereas scan mode, whether standard or high resolution, was less relevant (p 5 0.34). Conclusions: The smallest FOV available should always be used for endodontic applications, and it is not recommended to reduce the number of projections to 180. Using the standard scan mode instead of high resolution does not negatively influence the visibility of the root canal space and is therefore recommended.
Long-term sealing ability of root-canal sealer is of clinical importance. We compared the performance of five sealers and a dentine bonding agent, at thicknesses of 0.05 mm (thin layer) and 0.3 mm (thick layer), in 212 standard human root specimens, before and after storage in water for 2 years. Thick layers of Roth and Pulp Canal Sealer EWT (PCS-EWT) allowed more leakage than did thin layers (P = 0.0000, 0.0358 for Roth and PCS-EWT, respectively) whereas no significant difference was found between the thin and thick layers for AH26, Ketac-Endo, Sealapex and J&J Bonding Agent. The pooled results of the thin and thick layers, which have clinical relevance, showed that after storing the specimens in water for 2 years, AH26, Sealapex, Ketac-Endo and PCS-EWT leaked more than before storage in water (P = 0.0008, 0.0000, 0.0035, 0.0257 for AH26, Sealapex, Ketac-Endo and PCS-EWT respectively), and that J&J Bonding Agent allowed less leakage and PCS-EWT more leakage than the other four sealers (P = 0.0000).
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