Combinations of bacterial species in endodontic infections

Peters, L. B.; Pr, Wesselink; Winkelhoff, van Arie

doi:10.1046/j.1365-2591.2002.00550.x

Cited by 85 publications

(81 citation statements)

References 16 publications

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“…Culture studies have demonstrated that primary endodontic infections are characterized by a mixed consortium dominated by anaerobic bacteria and composed of a mean number of 2.6 to 5.4 taxa per canal (23,36,43,44). Nonetheless, broad-range molecular analyses of the root canal microbiota of teeth with chronic apical periodontitis have revealed higher figures: 7 taxa in denaturing gradient gel electrophoresis analyses (37), 11 taxa in terminal restriction fragment length polymorphism analyses (30), 10 to 12 taxa in clone library analyses (29,30), and 20 taxa in combined culturing and clone library analyses (20).…”

Section: Discussionmentioning

confidence: 99%

Root Canal Microbiota of Teeth with Chronic Apical Periodontitis

2008

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Samples from infected root canals of 43 teeth with chronic apical periodontitis were analyzed for the presence and relative levels of 83 oral bacterial species and/or phylotypes using a reverse-capture checkerboard hybridization assay. Associations between the most frequently detected taxa were also recorded. The most prevalent taxa were Olsenella uli (74%), Eikenella corrodens (63%), Porphyromonas endodontalis (56%), Peptostreptococcus anaerobius (54%), and Bacteroidetes oral clone X083 (51%). When prevalence was considered only for bacteria present at levels >10 5 , Bacteroidetes clone X083 was the most frequently isolated bacterium (37%), followed by Parvimonas micra (28%), E. corrodens (23%), and Tannerella forsythia (19%). The number of target taxa per canal was directly proportional to the size of the apical periodontitis lesion, with lesions >10 mm in diameter harboring a mean number of approximately 20 taxa. Several positive associations for the most prevalent taxa were disclosed for the first time and may have important ecological and pathogenic implications. In addition to strengthening the association of several cultivable named species with chronic apical periodontitis, the present findings using a large-scale analysis allowed the inclusion of some newly named species and as-yet-uncultivated phylotypes in the set of candidate pathogens associated with this disease.Chronic apical periodontitis is arguably one of the most common forms of biofilm-induced diseases that affect humans (9). The disease develops after dental pulp necrosis and infection as a result of caries, trauma, or iatrogenic clinical procedures. The environmental conditions in the necrotic root canal are conducive to the establishment of a microbiota conspicuously dominated by anaerobic bacteria. Bacterial profiles of the endodontic microbiota vary from individual to individual (37); i.e., each individual harbors a unique microbiota in terms of species richness and abundance. This indicates that apical periodontitis has a heterogeneous etiology, where no single species can be considered to be the main endodontic pathogen, and multiple bacterial combinations can play a role in disease causation.Early studies of the microbiota associated with apical periodontitis were conducted using broad-range culture methods. Those studies were followed by a generation of studies employing molecular detection methods such as species-specific PCR and the original checkerboard DNA-DNA hybridization assay to target cultivable bacteria previously isolated from infected canals or from other oral diseased sites. These methods allowed the inclusion of some culture-difficult species in the set of candidate endodontic pathogens. The adoption of 16S rRNA gene clone library analysis allowed an even more comprehensive broad-range investigation of bacterial communities in endodontic infections. By this technique, not only cultivable species but also as-yet-uncultivated and uncharacterized bacteria can be identified. Studies using the 16S rRNA gene clone library ana...

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Section: Discussionmentioning

confidence: 99%

Root Canal Microbiota of Teeth with Chronic Apical Periodontitis

2008

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“…5 Biomechanical treatment of root canals significantly reduces microbial organisms in the endodontic Declaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript. microbiota, 6 but it is unable to completely disinfect some areas due to anatomical complexities, causing persistent microorganisms to survive, multiply, and reinfect the pulp space. 7 Calcium hydroxide has been the most used intracanal medication due to its antimicrobial properties, promotion of a temporary physical sealing of the root canal, and osteogenic potential.…”

Section: Introductionmentioning

confidence: 99%

In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis

et al. 2015

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The aim of this study was to evaluate the in vitro antimicrobial activity of Brazilian brown propolis as an intracanal medication against Enterococcus faecalis. Thirty dentin discs prepared from intact freshly extracted bovine maxillary central incisors were infected with E. faecalis for 21 days. The specimens were distributed into six groups according to the medicament used as follows: G1-calcium hydroxide paste; G2-Carbowax 400 (control group); G3-20% brown propolis paste; G4-40% brown propolis paste; G5-20% brown propolis paste + calcium hydroxide paste; and G6-40% brown propolis paste + calcium hydroxide paste. The experimental pastes were placed into the canal lumen and left for 14 days. After each period, irrigation was performed with sterile saline to remove the medicament, and the canals were dried with sterile paper points. The dentin chips were removed from the canals with sequential sterile round burs at low speed and were immediately collected in separate test tubes containing BHI broth. The tubes were incubated at 37°C, and microbial growth was analyzed by spectrophotometry after 15 days. All the experimental medications significantly reduced the number of viable bacteria. The G4 and G5 pastes were more effective than the G1 paste, with 35.8%, 41%, and 21.3% antibacterial activity, respectively. Brazilian brown propolis shows antibacterial capacity against E. faecalis.

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“…12 Anaerobik Gram-negatif türler, Gram-pozitif anaeroplar, fakültatif ve mikroaerofilik streptokoklar endodontik enfeksiyonlardan sıklıkla izole edilen bakterilerdir. [19][20][21] Prevotella ve Porphyromonas grubunun bazı türleri, peptostreptokoklar, peptokoklar, Fusobacterium spp., Eubacterium spp. ve Actinomyces spp.…”

Section: 713unclassified

Endodonti̇k Enfeksi̇yonlarda Mi̇krobi̇oloji̇k Geneti̇k Tani Yöntemleri̇ni̇n Rolü

Özbek¹

2015

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Combinations of bacterial species in endodontic infections

Abstract: These results indicate that endodontic pathogens do not occur at random but are found in specific combinations.

Cited by 85 publications

References 16 publications

Root Canal Microbiota of Teeth with Chronic Apical Periodontitis

Root Canal Microbiota of Teeth with Chronic Apical Periodontitis

In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis

Endodonti̇k Enfeksi̇yonlarda Mi̇krobi̇oloji̇k Geneti̇k Tani Yöntemleri̇ni̇n Rolü

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