Objective: Huanglian Jiedu decoction (HLJDD) was shown to exert therapeutic effect on pneumonia for a long time in China. However, its pharmacological mechanism remains to be elucidated. Methods: The active compounds and target proteins of HLJDD were screened from TCMSP and the targets of pneumonia were obtained from GeneCards. GO and KEGG enrichment were applied in this study. Networks were established by Cytoscape with R-Bioconductor. The affinity between components and targets were detected by molecular docking. Finally, active ingredients and targets were selected to be verified in an inflammatory model established in LPS-induced A549 cells. CCK8 proliferation assay and western blot were performed to test the relative indicators. Results: 102 bioactive components and 205 targets from 4 herbs in HLJDD were collected. 68 potential therapeutic targets and 55 corresponding compounds were screened to establish the networks. 4 active compounds (quercetin, wogonin, kaempferol and baicalein) and 5 hub genes (IL6, AKT1, CXCL8, CCL2 and IL1B) were then selected to make molecular docking. The results indicated that quercetin and wogonin had better affinity with CXCL8, CCL2 or IL1B. In vitro experiments revealed that both quercetin and wogonin could decrease the proliferation inhibiting and apoptosis of A549 cells injured by LPS. The expression CXCL8, CCL2 or IL1B were down-regulated after quercetin or wogonin treating, compared with LPS-induced A549 cells (P < 0.01). Conclusion: The current study suggested that the mechanism of HLJDD treating pneumonia might be inhibiting the apoptosis through targeting the inflammatory factors mainly by quercetin and wogonin.
Background Invasive fungal disease (IFD) is associated with high morbidity and mortality. Data are lacking regarding physicians' perspectives on the diagnosis and management of IFD in China. Objectives To evaluate physicians' perspectives on the diagnosis and management of IFD. Methods Based on current guidelines, a questionnaire was designed and administered to 294 physicians working in haematology departments, intensive care units, respiratory departments and infectious diseases departments in 18 hospitals in China. Results The total score and subsection scores for invasive candidiasis, invasive aspergillosis (IA), cryptococcosis and invasive mucormycosis (IM) were 72.0 ± 12.2 (maximum = 100), 11.1 ± 2.7 (maximum = 19), 43.0 ± 7.8 (maximum = 57), 8.1 ± 2.0 (maximum = 11) and 9.8 ± 2.3 (maximum = 13), respectively. Although the perspectives of the Chinese physicians were in good overall agreement with guideline recommendations, some knowledge gaps were identified. Specific areas in which the physicians' perspectives and guideline recommendations differed included use of the β‐D‐glucan test to facilitate the diagnosis of IFD, relative utility of the serum galactomannan test and bronchoalveolar lavage fluid galactomannan test in patients with agranulocytosis, use of imaging in the diagnosis of mucormycosis, risk factors for mucormycosis, indications for initiating antifungal therapy in patients with haematological malignancies, when to start empirical therapy in mechanically ventilated patients, first‐line drugs for mucormycosis and treatment courses for IA and IM. Conclusion This study highlights the main areas that could be targeted by training programs to improve the knowledge of physicians treating patients with IFD in China.
The potential of long noncoding RNAs (lncRNAs) as symptomatic and prognostic biomarkers in oncotherapy for hepatic carcinoma has been revealed. However, the specific mechanisms of lncRNAregulated tumor progression and metastasis are not completely known. The RNA and miRNA sequencing data of liver cancer in The Cancer Genome Atlas database were analyzed. A competing endogenous RNA (ceRNA) was constructed, and its effects on the progression and metastasis of tumors and the survival rates of patients with tumor were evaluated. Differentially expressed mRNAs, miRNAs, and lncRNAs were identified. A liver cancer-associated ceRNA network, namely, FAM224A/hsa-mir-139/RAD54B, was then established. FAM224A/hsa-mir-139/RAD54B assumed a significant part in liver malignancy movement and metastasis. A series of cell experiments was conducted to prove the importance of ceRNAs, and the binding between RNAs was validated through a dual-luciferase reporter assay. The FAM224A/hsa-mir-139/RAD54B network in liver cancer was identified. FAM224A may be an important prognostic factor and treatment target for liver cancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.