Cancer-suppressing miR-520-3p gene inhibits proliferation, migration, and invasion of gastric cancer cells through targeted regulation of KLF7

Jiang, Wei; Yang, Wenjie; Li, Jun; Zhao, Xuequn; Lu, Wei

doi:10.1016/j.bulcan.2022.02.005

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…Matrigel matrix glue was added to the upper chamber of the invasion assay, but not add Matrigel matrix glue in the migration assay. The method was referred as the article ( 27 ). Finally, the cells were stained with 0.1% crystal violet for 20 min and observed with an Olympus BX51 microscope (Olympus, Tokyo, Japan) and counted with ImageJ software (US National Institutes of Health, Bethesda, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

KMO in the promotion of tumor development and progression in hepatocellular carcinoma

Xu¹,

Shen²,

Zeng³

et al. 2023

J Gastrointest Oncol

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Background Hepatocellular carcinoma (HCC) is one of the most common cancers and an important medical problem with poor prognosis. The role of messenger RNA (mRNA) has been broadly researched in the progression of different human cancers. Microarray analysis has demonstrated that kynurenine 3-monooxygenase ( KMO ) expression is lower in HCC, but the mechanism of KMO in regulating the development of HCC remains unknown. Methods Through comprehensive bioinformatics analysis of GSE101728 and GSE88839, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, protein-protein interaction (PPI) network analysis, gene expression, and overall survival (OS) analysis, KMO was selected as the candidate molecular marker in HCC. The expression of KMO at the protein and RNA level was evaluated by Western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR). Furthermore, the cell proliferation, migration, invasion, and apoptosis, and the protein levels of epithelial-mesenchymal transition (EMT) markers were examined with Cell Counting Kit 8 (CCK-8) assays, Transwell assay, flow cytometry, and WB. Results Through comprehensive bioinformatics analysis, we found that the low expression of KMO in HCC is not conducive to a good prognosis of HCC. Then, through in vitro cell experiments, we found that low expression of KMO promoted HCC proliferation, invasion, metastasis, EMT, and cell apoptosis. Additionally, hsa-miR-3613-5p was found to be highly expressed in HCC cells and could negatively regulate the expression of KMO. Moreover, hsa-miR-3613-5p was found to be the target microRNA (miRNA) of KMO according to qRT-PCR verification. Conclusions KMO plays an important role in the early diagnosis, prognosis, occurrence, and development of liver cancer, and may target miR-3613-5p to function. This represents a novel insight into understanding the molecular mechanisms of HCC.

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Section: Methodsmentioning

confidence: 99%