Soil salinity can be a limiting factor for productivity in agriculture and forestry. In order to fully utilize saline lands productively in plantation forestry for pulp production, the genetic modification of tree species for salttolerance may be required. The AhDREB1 gene, a DREBlike transcription factor gene, was transferred into Populus tomentosa by Agrobacterium-mediated transformation. Transgenic plants were regenerated and selected using a two-step process; first on Murashige and Skoog (MS) basal medium containing 4.44 lM 6-benzyladenine (BA), 1.61 lM a-naphthaleneacetic acid (NAA), 30 mg l -1 kanamycin, and 250 mg l -1 ceftomine, and then enhanced selection on medium with 50 mg l -1 kanamycin. The putative transformants were confirmed by polymerase chain reaction (PCR) and Southern hybridization for the AhDREB1 gene. Transgenic plants were rooted on halfstrength MS medium containing 5.71 lM indole-3-acetic acid (IAA), 1.61 lM NAA, 2 g l -1 sucrose, and 5 g l -1 agar. The salt tolerance of transgenic plants in pots in the greenhouse showed a survival rate of 100, 100, 84.4, and 44.4% after watering with a solution of 34.2, 68.4, 102.7, and 136.9 mM NaCl, respectively.
Intractable vertigo syndrome can be diagnosed after failure of routine treatments for benign paroxysmal positional vertigo (BPPV). Stellate ganglion block can be used in patients who do not respond to oral medication. However, buccal acupuncture combined with stellate ganglion block in the treatment of intractable vertigo syndrome has not been described yet. In this case report, we describe a patient who showed good response to combined treatment with buccal acupuncture and stellate ganglion block
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