In the last decades, surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) has attracted increasing interest due to its unique capabilities, achievable through the nanostructured substrates used to promote the analyte desorption/ionization. While the most widely recognized asset of SALDI-MS is the untargeted analysis of small molecules, this technique also offers the possibility of targeted approaches. In particular, the implementation of SALDI-MS imaging (SALDI-MSI), which is the focus of this review, opens up new opportunities. After a brief discussion of the nomenclature and the fundamental mechanisms associated with this technique, which are still highly controversial, the
Surface-enhanced Raman scattering (SERS) nanoprobes based on Au@Ag core@shell nanoparticles coated with poly(allylamine) were functionalized with small targeting molecules to evaluate simultaneously the level of expression of two cancer-related markers, both in cells and in tissues. The Au@Ag nanoparticles provide a high SERS signal enhancement in the visible range when combined with resonant Raman-active molecules. The poly(allylamine) coating plays a dual key role in (i) protecting the metal surface against the complex biological medium, leading to a stable signal of the Raman-active molecules, and (ii) enabling specific biofunctionalization through its amine functions. Using small targeting molecules linked to the polymer coating, two different nanoprobes (duplex approach) were designed. Each was able to specifically target a particular cancerrelated marker: folate receptors (FRs) and sialic acid (SA). We demonstrate that the level of expression of these targeted markers can be evaluated following the SERS signal of the probes incubated on cells or tissues. The potential overexpression of folate receptors and of sialic acid was evaluated and measured in breast and ovarian cancerous tissue sections. In addition, FR and/or SA overexpression in the tumor region can be visualized with high contrast with respect to the healthy region and with high spatial accuracy consistent with histology by SERS imaging of the nanoprobe signal. Owing to the unique spectral signature of the designed nanoprobes, this approach offers an efficient tool for the spatially resolved, in situ measurement of the expression level of several cancer-related markers in tumors at the same time.
While the imaging of bacteria, directly on agar-based media, has been reported in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI), such samples usually require laborious and time-consuming preparation prior to analysis. Furthermore, the MALDI sample preparation may require desiccation, potentially causing sample deformation and/or degradation. Other issues may be associated with the use of a matrix in MALDI-MSI. Here, we propose an alternative approach to image a co-culture of Pseudomonas and Bacillus bacteria, grown on agar, in the positive and negative ionization modes, using surface-assisted laser desorption/ionization (SAL-DI) MSI. A rapid and easy sample preparation was performed using a "desorption/ionization using through-hole alumina membrane" (DIUTHAME) with a blotting method. The porous DIUTHAME membrane was used (1) for the transfer of the metabolites from the sample to the membrane, and (2) as assisting material in SALDI-MSI. The advantages and limitations of this new sample preparation are discussed in this paper.
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