Imaging lipids in biological samples with surface-assisted laser desorption/ionization mass spectrometry: A concise review of the last decade

Müller, Wendy; Pauw, Edwin De; Far, Johann; Malherbe, Cédric; Eppe, Gauthier

doi:10.1016/j.plipres.2021.101114

Cited by 28 publications

(25 citation statements)

References 151 publications

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“…[16,20] Third, the ionization and fragmentation of the MALDI matrix usually produce strong interference signals in the low m/z range, overlapping or suppressing the signals of low m/z ions (< 1000 Da). [3,16] Thus, to overcome the aforementioned challenges, alternative methods (dealing with sample preparation and requiring no addition of an organic matrix) are desirable, given the growing interest in the analysis of microbial samples using MSI.…”

Section: Introductionmentioning

confidence: 99%

“…Mass spectrometry imaging (MSI) has become a powerful technology, allowing the spatial mapping of a wide range of biological (e. g. small metabolites, [1,2] lipids, [3][4][5] proteins, [6,7] peptides) and non-biological (e. g. drugs) [8,9] molecules in complex systems, such as tissues, [10][11][12][13] cells and bacterial colonies. [14,15] Among its broad applications, MSI can be used to investigate the distribution of metabolites produced by microbial species.…”

Section: Introductionmentioning

confidence: 99%

“…[24,[27][28][29] SALDI-MSI offers several advantages over MALDI-MSI, such as a clear background in the low m/z range enabling the analysis of small molecules, a higher signal reproducibility, and the ability to carry out analyses in both ionization modes with the same nanostructured substrate. [3,24,30,31] SALDI-MS has already been used with the aim of discovering new bioactive compounds excreted by microorganisms, including fungi [32] and molds. [33] In particular, SALDI-MSI imprinting methods have been used to image the metabolic interactions between microbial colonies, using nanostructured silicon substrates [16,34] and TiO 2 nanowires, [35] for example.…”

Section: Introductionmentioning

confidence: 99%

“…Second, the center of the microbial colony may absorb more MALDI matrix than the colony edges or surrounding agar medium, leading to uneven matrix coating, which generates signal artifacts (such as little to no signal within the bacterial colony) [16,20] . Third, the ionization and fragmentation of the MALDI matrix usually produce strong interference signals in the low m/z range, overlapping or suppressing the signals of low m/z ions (< 1000 Da) [3,16] . Thus, to overcome the aforementioned challenges, alternative methods (dealing with sample preparation and requiring no addition of an organic matrix) are desirable, given the growing interest in the analysis of microbial samples using MSI.…”

Section: Introductionmentioning

confidence: 99%

“…One of the alternative workflows includes surface‐assisted laser desorption/ionization (SALDI) MSI [3,24–26] combined with an imprinting or blotting sample preparation in which the analytes are transferred from the sample to an appropriate support prior to analysis [24,27–29] . SALDI‐MSI offers several advantages over MALDI‐MSI, such as a clear background in the low m/z range enabling the analysis of small molecules, a higher signal reproducibility, and the ability to carry out analyses in both ionization modes with the same nanostructured substrate [3,24,30,31] . SALDI‐MS has already been used with the aim of discovering new bioactive compounds excreted by microorganisms, including fungi [32] and molds [33] .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Imaging Metabolites in Agar‐Based Bacterial Co‐Cultures with Minimal Sample Preparation using a DIUTHAME Membrane in Surface‐Assisted Laser Desorption/Ionization Mass Spectrometry**

et al. 2022

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While the imaging of bacteria, directly on agar-based media, has been reported in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI), such samples usually require laborious and time-consuming preparation prior to analysis. Furthermore, the MALDI sample preparation may require desiccation, potentially causing sample deformation and/or degradation. Other issues may be associated with the use of a matrix in MALDI-MSI. Here, we propose an alternative approach to image a co-culture of Pseudomonas and Bacillus bacteria, grown on agar, in the positive and negative ionization modes, using surface-assisted laser desorption/ionization (SAL-DI) MSI. A rapid and easy sample preparation was performed using a "desorption/ionization using through-hole alumina membrane" (DIUTHAME) with a blotting method. The porous DIUTHAME membrane was used (1) for the transfer of the metabolites from the sample to the membrane, and (2) as assisting material in SALDI-MSI. The advantages and limitations of this new sample preparation are discussed in this paper.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Imaging Metabolites in Agar‐Based Bacterial Co‐Cultures with Minimal Sample Preparation using a DIUTHAME Membrane in Surface‐Assisted Laser Desorption/Ionization Mass Spectrometry**

et al. 2022

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Study of sample preparation influence on bacterial lipids profile in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Arendowski,

Sibińska,

Miśta

et al. 2023

Lipids

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Lipids are one of the cell components therefore it is important to be able to accurately assess them. One of the analytical techniques used to study lipid profiles is matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI TOF MS). The present study attempted to select optimal conditions for sample preparation and MALDI MS analysis of bacterial lipidome in both positive and negative ion modes using different extraction protocols—Folch, Matyash, and Bligh & Dyer, solvents used to apply samples, and matrices such as 9‐aminoacridine (9‐AA), α‐cyano‐4‐hydroxycinnamic acid (CHCA), 2,5‐dihydroxybenzoic acid (DHB), 2‐mercaptobenzothiazole (MBT), and 2,4,6‐trihydroxyacetophenone (THAP). The obtained results allowed concluding that DHB or CHCA matrices are suitable for lipid analysis in the positive mode, and in the negative mode THAP or 9‐AA. The most appropriate protocol for extracting lipids from bacterial cells was the Bligh & Dyer method in both ionization modes. The use of the solvent TA30, which was a mixture of acetonitrile and 0.1% trifluoroacetic acid in water, provided on the spectra a significant number of signals from lipids in all groups analyzed, such as fatty acyls, glycerolipids, and glycerophospholipids.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Imaging lipids in biological samples with surface-assisted laser desorption/ionization mass spectrometry: A concise review of the last decade

Cited by 28 publications

References 151 publications

Imaging Metabolites in Agar‐Based Bacterial Co‐Cultures with Minimal Sample Preparation using a DIUTHAME Membrane in Surface‐Assisted Laser Desorption/Ionization Mass Spectrometry**

Imaging Metabolites in Agar‐Based Bacterial Co‐Cultures with Minimal Sample Preparation using a DIUTHAME Membrane in Surface‐Assisted Laser Desorption/Ionization Mass Spectrometry**

Study of sample preparation influence on bacterial lipids profile in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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