Ferroptosis is a newly discovered form of regulated cell death dependent on iron and reactive oxygen species (ROS). It directly or indirectly affects the activity of glutathione peroxidases (GPXs) under the induction of small molecules, causing membrane lipid peroxidation due to redox imbalances and excessive ROS accumulation, damaging the integrity of cell membranes. Ferroptosis is mainly characterized by mitochondrial shrinkage, increased density of bilayer membranes, and the accumulation of lipid peroxidation. Myocardial ischemia-reperfusion injury (MIRI) is an unavoidable risk event for acute myocardial infarction. Ferroptosis is closely associated with MIRI, and this relationship is discussed in detail here. This review systematically summarizes the process of ferroptosis and the latest research progress on the role of ferroptosis in MIRI to provide new ideas for the prevention and treatment of MIRI.

In this study, we aim to investigate the regulation of specific long non-coding RNAs (lncRNAs) on the progression of ischemia/reperfusion (I/R) injury. We identified and characterized the exosomes derived from mouse primary aortic endothelial cells. Subsequently, we found that these exosomes expressed typical exosomal markers and high levels of LINC00174, which significantly ameliorated I/R-induced myocardial damage and suppressed the apoptosis, vacuolation, and autophagy of myocardial cells. Mechanistic approaches revealed that LINC00174 directly interacted with SRSF1 to suppress the expression of p53, thus restraining the transcription of myocardin and repressing the activation of the Akt/AMPK pathway that was crucial for autophagy initiation in I/R-induced myocardial damage. Moreover, this molecular mechanism was verified by in vivo study. In summary, exosomal LINC00174 generated from vascular endothelial cells repressed p53-mediated autophagy and apoptosis to mitigate I/R-induced myocardial damage, suggesting that targeting LINC00174 may be a novel strategy to treat I/R-induced myocardial infarction.

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Hands-on learning is proposed as a prerequisite for mathematics learning in kindergarten and primary school. However, it remains unclear that whether hands-on experience aids understanding of geometry knowledge for middle school students. We also know little about the neural basis underlying the value of hands-on experience in math education. In this study, 40 right-handed Chinese students (20 boys and 20 girls) with different academic levels were selected from 126 seventh-grade students in the same school, who learnt “Axisymmetric of an Isosceles Triangle” in different learning style (hands-on operation vs. video observation). Half of them operated the concrete manipulatives while the other half watched the instructional videos. The learning-test paradigm and functional near-infrared spectroscopy (fNIRS) technique were used to compare the differences in geometry reasoning involved in solving well-structured problems and ill-structured problems. Behavioral results showed that hands-on experience promoted students’ performances of geometry problem-solving. Students with lower academic level were more dependent on hands-on experience than those with higher academic level. The fNIRS results showed that meaningful hands-on experience with concrete manipulatives related to learning contents increased reactivation of the somatosensory association cortex during subsequent reasoning, which helped to improve the problem-solving performance. Hands-on experience also reduced students’ cognitive load during the well-structured problem-solving process. These findings contribute to better understand the value of hands-on experience in geometry learning and the implications for future mathematics classroom practices.

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