Objective This study aimed to identify the hub genes and pathways of genes related to oxidative stress of cartilage in osteonecrosis of femoral head (ONFH), and to predict the transcription factors of the hub genes. Methods The GSE74089 was obtained from the Gene Expression Omnibus (GEO) database, including 4 necrotic tissues and 4 normal tissues, and the differentially expressed genes (DEGs) were identified by limma package in R language. Simultaneously, we searched for the genes related to oxidative stress in the Gene Ontology (GO) database. GO and signaling pathways analysis were performed using DAVID, Metascape, and GSEA. Protein-protein interaction (PPI) network was constructed using the STRING database, and the Degree algorithm of Cytoscape software was used to screen for hub genes. Finally, the NetworkAnalyst web tool was used to find the hub genes’ transcriptional factors (TFs). Results In total, 440 oxidative stress–related genes were found in GSE74089 and GO database, and 88 of them were significantly differentially expressed. These genes were mainly involved in several signaling pathways, such as MAPK signaling pathway, PI3K-AKT-mTOR signaling pathway, FOXO signaling pathway. The top 10 hub genes were JUN, FOXO3, CASP3, JAK2, RELA, EZH2, ABL1, PTGS2, FBXW7, MCL1. Besides, TFAP2A, GATA2, SP1, and E2F1 may be the key regulatory factors of hub genes. Conclusions We identified some hub genes and signaling pathways associated with oxidative stress in ONFH through a series of bioinformatics analyses.

The purpose of this meta‐analysis was to identify if patient‐specific instrumentation (PSI) could increase the accuracy of the correction in high tibial osteotomy (HTO) and to explore the assessment indices and the necessity of using a PSI in HTO. A systematic search was carried out using online databases. A total of 466 patients were included in 11 papers that matched the inclusion criteria. To evaluate the accuracy of PSI‐assisted HTO, the weight bearing line ratio (WBL%), hip‐knee‐ankle angle (HKA), mechanical medial proximal tibial angle (mMPTA), and posterior tibial slope angle (PTSA) were measured preoperatively and postoperatively and compared to the designed target values. Statistical analysis was performed after strict data extraction with Review Manager (version 5.4). Significant differences were detected in WBL% (MD = −36.41; 95% CI: −42.30 to −30.53; p < 0.00001), HKA (MD = −9.95; 95% CI: –11.65 to –8.25; p < 0.00001), and mMPTA (MD = –8.40; 95% CI:−10.27 to −6.53; p < 0.00001) but not in PTSA (MD = 0.34; 95% CI: −0.59 to 1.27; p = 0.47) between preoperative and postoperative measurements. There was no significant difference between the designed target values and the postoperative correction values of HKA (MD = 0.14; 95% CI: −0.19 to 0.47; p = 0.41) or mMPTA (MD = 0.11; 95% CI −0.34 to 0.55; p = 0.64). The data show that 3D‐based planning of PSI for HTO is both accurate and safe. WBL%, HKA, and mMPTA were the optimal evaluation indicators of coronal plane correction. Sagittal correction is best evaluated by the PTSA. The present study reports that PSI is accurate but not necessary in typical HTO.

Clear cell renal cell carcinoma (ccRCC) is a primary malignant tumour of tubular epithelial origin and is most common in the urinary tract. Growing evidence suggests that oxidative stress (OS), generates high levels of reactive oxygen species (ROS) and free radicals, and plays a critical role in cancer in humans. However, the predictive value of OS-related long non-coding RNAs (lncRNAs) in ccRCC remains unclear. We constructed a predictive signature of survival based on OS-related lncRNAs that were obtained from The Cancer Genome Atlas (TCGA–KIRC), to predict the prognosis of patients with ccRCC. The signature comprised seven lncRNAs: SPART-AS1, AL162586.1, LINC00944, LINC01550, HOXB-AS4, LINC02027, and DOCK9-DT. OS-related signature of lncRNAs had diagnostic efficiency higher than that of clinicopathological variables, with an area of 0.794 under the receiver operating characteristic curve. Additionally, the nomogram based on risk scores and clinicopathological variables (age, gender, grade, stage, M-stage, and N-stage) showed strong predictive performance. Patients with high-risk were found to be more sensitive to the therapeutic drugs ABT.888, AICAR, MS.275, sunitinib, AZD.2281, and GDC.0449. Our constructed the predictive signature can independently predict the prognosis of patients with ccRCC; however, the underlying mechanism needs further investigation.

Purpose: To determine whether there is a causal effect of inflammatory bowel disease (IBD) on ocular inflammation? Design: Two-sample Mendelian randomization (MR) study. Methods: IBD-associated genetic instruments were derived from the largest genome-wide association studies published to date for IBD, ulcerative colitis (UC), and Crohn’s diseases (CD). FinnGen research project was used to identify genetic risk variants for conjunctivitis, keratitis, iridocyclitis, chorioretinitis, episcleritis, and optic neuritis. All participants were of European ancestry. Inverse-variance−weighted (IVW) was used as the primary outcome, while weighted median (WM) and MR-Egger were used to improve the estimation of IVW. Results: A nominal causal effect of genetically predicted IBD on risk of conjunctivitis, keratitis, iridocyclitis, and optic neuritis, but not on chorioretinitis or episcleritis. After Bonferroni correction, the results showed that genetically predicted UC was significantly associated with an increased risk of iridocyclitis (IVW: OR, 1.17; 95% CI, 1.10-1.24, P=2.54×10-7), CD was significantly associated with conjunctivitis (IVW: OR, 1.05; 95% CI, 1.03-1.08, P=3.20×10-5), keratitis (IVW: OR, 1.06; 95% CI, 1.02-1.09; P=1.13×10-3), and iridocyclitis (IVW: OR, 1.09; 95% CI, 1.04-1.14; P=1.43×10-4). Conclusion: This study illustrates that IBD causally poses a risk of inflammation of conjunctival, cornea, iris-ciliary，and optic neuritis. Moreover, CD is more closely associated with the eye than UC. These implyed that the relationship of IBD and different parts of the eye structure were different, and provided novel evidence linking based on the association of the gut-eye axis.

Background: Starving intratumoral microenvironment prominently alters genic profiles including long noncoding RNAs (lncRNAs), which further regulate bladder cancer (BCa) malignant biological properties, such as invasion and migration. Methods: Transcriptome RNA-sequencing data of 414 BCa tumor tissues and 19 normal tissues were obtained from TCGA database and paired samples of 132 BCa patients. A chain of in vitro validations such as qPCR, migration and invasion assays were performed to reveal the clinical relevance of AC011472.4 and AL157895.1. Results: A total of 11 lncRNAs were identified as starvation-related lncRNAs, of which AC011472.4 and AL157895.1 were relevant to overall survival of BCa patients. Besides, a starvation-related risk score model was established based on the levels of AC011472.4 and AL157895.1. BCa patients with higher levels of AL157895.1 were divided into the high-risk group and usually obtained higher mortality rate, but AC011472.4 was contrary. AL157895.1 expressed highly in BCa cell lines and tumour tissues, especially in patients with the advanced grade, stage and T-stage, while AC011472.4 showed the reversed result. Moreover, increased level of AL157895.1 was remarkably correlated to T-stage, muscle invasion status and distant metastasis. SiRNAsmediated silence of AC011472.4 and AL157895.1 respectively increased and diminished invasion and migration properties of BCa cells. Conclusions: In this study, we highlight the significant roles of AC011472.4 and AL157895.1 on evaluating prognoses of BCa patients and validate their correlation with various clinical parameters. These findings provide an appropriate risk score model for BCa clinical decision making.

Clear cell renal carcinoma (ccRCC) is a primary malignant tumor of tubular epithelial origin that is most common in the urinary tract. Growing evidence suggests that oxidative stress (OS), which generates high levels of reactive oxygen species (ROS) and free radicals, plays a critical role in human cancer. However, the predictive value of OS-related lncRNAs in ccRCC remains unclear. We constructed a new survival signature from OS-related and diffeomorphic lncRNAs obtained from the Cancer Genome Atlas (TCGA-KIRC). The signature comprised seven lncRNAs: SPART-AS1, AL162586.1, LINC00944, LINC01550, HOXB-AS4, LINC02027, and DOCK9-DT. OS-related lncRNA signatures had higher diagnostic efficiency than clinicopathological variables, with an area of 0.794 under the receiver operating characteristic curve. Additionally, nomograms based on risk scores and clinicopathological variables (age, gender, grade and stage) showed strong predictive performance. High-risk patients are more sensitive to the therapeutic drugs bicalutamide, epothilone, and lapatinib. Our signatures can independently predict the prognosis of patients with ccRCC; however, the mechanism there of needs further investigation.

the effect of dynamic change activities of enzymatic in Sugarcane field with different coating material was studied With three kinds of coated compound fertilizer as raw materials. Results show that the soil enzymatic activities of catalase,sucrase and urease increased first and then decreased at different sugarcane growth stage; By comparing the Coated compound fertilizer of coated materials ofIdesia polycarpavar.vestitawith CK, we realized the quantity of soil catalase increased 0.03mol/L、that of soil sucrase increased 0.91 mg/g、that of soil urease increased 0.05 mg/g and that of the production increased 23.7%. That was beneficial to the improvement of soil enzyme activity of coated compound fertilizer as raw materials oil ofIdesia polycarpa var.vestita.

Background: QuantiFERON-TB Gold Plus (QFT-Plus) is an important test that has emerged in recent years for detecting TB infection. We conducted a review to compare the sensitivity, specificity and positive rate of QFT-Plus with that of QuantiFERON-TB Gold In-Tube (QFT-GIT), T-cell spot of tuberculosis assay (T-SPOT.TB) and Tuberculin test (TST). Methods: PubMed and Embase were searched, without language restrictions, from 1 January 2015 to 31 March 2022 using “Mycobacterium tuberculosis Infections” and “QuantiFERON-TB-Plus” etc. We estimated the sensitivity from studies of patients with active tuberculosis, specificity from studies of populations with very low risk of TB exposure, and positive rate from studies of high-risk populations. The methodological quality of the eligible studies was assessed, and a random-effects model meta-analysis was used to determine the risk difference (RD). We assessed the pooled rate by using a random-effects model. PROSPERO number was CRD 42021267432. Results: Of 3996 studies, 83 were eligible for full-text screening and 41 were included in the meta-analysis. In patients with active TB, the sensitivity of QFT-Plus was compared to that of QFT-GIT and T-SPOT.TB, respectively, and no statistically significant differences were found. In populations with a very low risk of TB exposure, the sensitivity of QFT-Plus was compared with that of QFT-GTI and T-SPOT.TB, respectively, and no statistically significant differences were found. Two studies were eligible to compare the specificity of the QFT-Plus test with that of the TST test, and the pooled RD was 0.12 (95% CI 0.02 to 0.22). In high-risk populations, 17 studies were eligible to compare the positive rate of the QFT-Plus test with that of the QFT-GIT test, and the pooled RD was 0.02 (95% CI -0.02 to 0.03). The positive rate of QFT-Plus was compared with that of T-SPOT.TB and TST groups, and no statistically significant differences were found. Conclusions: The diagnostic performance of QFT-Plus was similar to that of QFT-GIT and T-SPOT.TB, but was slightly more specific than TST.