Furazolidone is a broad-spectrum antibiotic that is frequently used in aquaculture on account of its excellent antibacterial properties. In this study, both the enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods were used to analyze the content of residual 3-amino-2-oxazolidinone (AOZ), a metabolite of furazolidone in Tilapia tissue. Homogenized fish samples were spiked with various amounts of AOZ, and following combined acid-hydrolysis and derivatization of the homogenized tissue with 2-NBA (2-nitrobenzaldehyde), sample clean-up was performed and the derived 2-nitrophenylmethylene-3-amino-2-oxazolidinone (NPAOZ) was analyzed. Using the LC-MS/MS method, a linear correlation between measured concentration Y and spiked concentration X was observed: Y = 0.4518X -0.0166, R 2 = 0.9972. The linear equation for the ELISA method was Y = 0.9322X + 0.5168, R 2 = 0.9066. These results demonstrated that the ELISA method might overestimate the residual AOZ content at low concentrations. The detection limit and recovery of the known addition were 0.05 mg kg -1 and 108% for the LC-MS/MS method and 0.31 mg kg -1 and 305% for the ELISA method, respectively.
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